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Citations for GE Life Sciences :
201 - 250 of 552 citations for Recombinant Human DCN Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Fc-fused sdAbs were prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE and the Western blot using standard protocols for dimerization ...
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bioRxiv - Microbiology 2021Quote: ... Fc-fused nanobody was prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE using standard protocols for dimerization ...
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bioRxiv - Immunology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare) and cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare). The protein was cleaved using bovine enterokinase (GenScript ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Microbiology 2020Quote: ... The rS1-Fc was protein A purified using MabSelect SuReLX resin (# 1754702) obtained from GE Healthcare.
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bioRxiv - Biophysics 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Immunology 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Immunology 2023Quote: ... and Fc using size exclusion chromatragraphy with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated using 100 kDa Amicon concentrators ...
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bioRxiv - Biochemistry 2021Quote: ... Both untagged and His-tagged Impβ were further purified using a Superdex 200 16/60 gel filtration column (GE Healthcare) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Microbiology 2021Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer (50 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant containing the 6xHis-tagged DMTases was stirred with 6 mL of NiCl2-charged chelating Sepharose beads (GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...