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Citations for GE Life Sciences :
151 - 200 of 1208 citations for Recombinant Human CSNK1G1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Plant Biology 2022Quote: ... The GST-MIK1CD is loaded onto GST agarose (Glutathione Sepharose 4B, GE Healthcare) and eluted using GST elution buffer containing 50 mM Tris–HCl ...
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bioRxiv - Plant Biology 2023Quote: ... GST and GST-CTU2 were coupled to Glutathione beads (GE Healthcare Life Sciences) and then were incubated with ROL5-His in the binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Cell Biology 2019Quote: Binding affinities were measured using purified and immobilized GST-SH3Boi1 or GST-SH3Boi2 as ligands on an anti-GST chip on a Biacore X100 device (GE Healthcare, Freiburg, Germany). HBSEP buffer was used as running buffer in all experiments ...
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bioRxiv - Biochemistry 2021Quote: ... GST-SUMO2 and GST-diSUMO2 were purified using glutathione sepharose 4B beads (GE Healthcare) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the GST-YTHDF1 and GST were purified with glutathione beads (GE Healthcare). The resulting purified GST-YTHDF1 or GST was incubated with Myc-STUB1 purified from HEK293T cells ...
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bioRxiv - Developmental Biology 2023Quote: ... GST or GST-importins were allowed to bind to glutathione-Sepharose 4B (GE Healthcare). In a typical experiment 100 μl beads were pre-equilibrated in IP-buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... GST fusion proteins or GST alone were bound on glutathione-Sepharose resin (GE Healthcare) and incubated with 5 μg of purified Snu71 or BBP protein in pull-down buffer (20 mM Tris pH 8.0 ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... goat anti-GST (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... GST (GE Healthcare, IL, USA), total tau (total human tau ...
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bioRxiv - Cell Biology 2019Quote: ... goat GST (GE healthcare 27457701), rabbit His (Abcam ab9108) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-GST (GE Healthcare, #27457701V) and anti-goat-HRP (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Plant Biology 2022Quote: ... The bait protein GST-WL1 was incubated with GST beads (GE Healthcare, Chicago, IL, USA) in non-EDTA buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-AtEPL1A and GST-AtEPL1B were purified on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... GST-TRAF6RING and GST-RNF38RING (residues 387-465) were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Microbiology 2020Quote: ... GST-E1 and GST-PknG were removed from the reactions with glutathione-Sepharose 4B (GE Healthcare). Next ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Developmental Biology 2020Quote: ... or GST Sepharose beads (GE Healthcare). GST pull-down was performed as described previously (Wei et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL GST beads (GE Healthcare) were added to the system for 2 h on a rotor at 4 °C.
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bioRxiv - Cell Biology 2022Quote: ... anti-GST (GE Healthcare, Cat# 27457701), anti-HA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... GST (GE Healthcare, 27-4577-01), and secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... or GST agarose resin (GE Healthcare), and purified via size-exclusion chromatography using a Superdex 200 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: GST-Byr2 (65-180) and GST-Scd1 (760-872) were purified using glutathione sepharose 4B (GE Healthcare). The proteins bound on the glutathione beads were washed with buffer A and used for the GST pull-down assays together with the Ras1.G17V (1-172 ...
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bioRxiv - Microbiology 2023Quote: ... The fusion construct GST SifAWT or GST SifAK11R were purified by using glutathione sepharose beads (GE Healthcare) and further cleaved with thrombin for tag separation ...
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bioRxiv - Molecular Biology 2023Quote: ... and GST-G3BP1-ΔIDR3 (deleting E411-Q466) was then loaded on a GST HP column (GE Healthcare) equilibrated with GSTBufferA (50 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Purified GST-Slp1(456-488aa) was detected with either goat anti-GST HRP-conjugated antibodies (GE Healthcare) or rabbit polyclonal anti-pThr480 antibodies.
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Cdc11 and GST alone were immobilized on 100 µl equilibrated Glutathione sepharose slurry (GE Healthcare, Freiburg, Germany) directly from the crude extract ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GST (GE Healthcare, 27-4577-01); anti-G9a (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... once loaded GST-PreScission Protease (GE Healthcare) was injected into the column and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST (27-4577-01; GE Healthcare). Secondary antibodies were purchased from Li-cor Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... The pGEX-4T-1 (GST) (GE Healthcare) and pMAL-c5x (MBP ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... KIX domain was cleaved from GST by incubation of resin-bound GST-KIX with 160 U thrombin (GE Healthcare) overnight at room temperature ...