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Citations for GE Life Sciences :
201 - 250 of 1074 citations for Recombinant Human CD3e Molecule Epsilon CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RBD:2-15 complex was then isolated by gel filtration on Superdex-200 (GE Healthcare). Fractions containing complexes were pooled and concentrated to 6.5 mg/ml in SEC buffer ...
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bioRxiv - Biophysics 2022Quote: ... The complex was loaded on a Superose 6 10/300 column (GE healthcare, currently Cytiva) pre-equilibrated with 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: Binding properties of OT-I TCR interaction with OVA were measured by SPR on a Biacore S200 and T200 (GE Healthcare Life Sciences) using a CM5 sensor chip and by GCI on the WAVEsystem (Creoptix ...
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bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE healthcare). Histones were eluted by a salt gradient from 200 mM to 600 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
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bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
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bioRxiv - Physiology 2019Quote: ... GST-tagged protein from supernatant were purified using glutathione-Sepharose 4B (GE Healthcare). Sepharose bound proteins were used as the protein columns ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Biophysics 2021Quote: ... The MtaLonA-Ig2-MMH8709-ATP-γ-S complex was then loaded onto Superdex 200 (GE Healthcare) column pre-equilibrated with 20 mM Tris-HCl (pH8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... the complex was further purified on a Superdex Increase 200 10/300 GL column (GE Healthcare) in separation buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The SpaO/SpaOC/OrgB complex was immobilized on HisTrap HP columns (GE Healthcare, Chicago, IL, USA), washed with buffer B3 containing 3 mM ATP and 10 mM MgCl2 and eluted with buffer B3 containing 400 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The assembled 20S proteasome complex was loaded onto a Superdex 200 10/300 GL (GE Healthcare) pre-equilibrated in 50 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex were then applied onto a 5 ml HiTrap Heparin column (GE Healthcare), and after washing with 5 CV of lysis buffer the bound material was eluted with 4 CV of Heparin elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The RNP complex was further purified with a Superose6 Increase 10/300 GL column (GE Healthcare), eluting into SEC Buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Complex samples they were SEC purified using a Superose 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
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bioRxiv - Immunology 2020Quote: ... The complex was SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column in TBS ...
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bioRxiv - Cell Biology 2019Quote: ... the harvested Ska complex was further purified by anion exchange chromatography (HiTrap Q FF, GE Healthcare) and gel filtration chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... The complex was SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column in TBS ...
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bioRxiv - Immunology 2020Quote: ... The complex was SEC purified using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) column in TBS ...
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bioRxiv - Immunology 2020Quote: ... The complex was SEC purified using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) column in TBS ...
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bioRxiv - Biochemistry 2021Quote: ... the complex was further purified by Heparin and HiLoad 16/600 Superdex 200 columns (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The complex was purified using a Superdex 200 Increase 3.2/300 gel filtration column (GE Healthcare) in a buffer comprising 10 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... The complex was isolated by size-exclusion chromatography (SEC) (Superdex 200 Increase 10/300, GE Healthcare) in 1xHBS buffer (10 mM Hepes-NaOH pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... the protein-DNA complex was further purified using Hiload 16/60 Superdex 75 column (GE Healthcare). Then ...
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bioRxiv - Biochemistry 2023Quote: ... The FoxP3ΔN-DNA complex was then purified by Superose 6 Increase 10/300 GL (GE Healthcare) column in 20mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabelled molecules were analysed by phosphorimaging using a Typhoon FLA 9500 and quantified by densitometry using ImageQuant software (GE Healthcare). In experiments employing an X0 HJ with one SP in strand X0-3 (X0-SP) ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was fractionated using a Hi-Trap Ni2+ column (GE Healthcare) following the manufacturer’s instructions and checked by SDS-PAGE electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
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bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...