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Citations for GE Life Sciences :
201 - 250 of 1146 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tagged TMIGD1 constructs in pGEX-4T-1 (GE Healthcare): GST-TMIGD1 (hTMIGD1 cytoplasmic tail (AA 242-262) ...
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bioRxiv - Developmental Biology 2019Quote: GST-tagged protein and bounded by Glutathione beads (GE healthcare). 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3) ...
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... followed by the removal of excess label-molecules by a gravity spin column (GE Healthcare, Germany).
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bioRxiv - Immunology 2021Quote: ... TCR and pMHC proteins were dialyzed and further purified via anion exchange chromatography (HiTrap Q HP; Mono Q; GE Healthcare) and size-exclusion (Superdex 200 ...
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bioRxiv - Biochemistry 2019Quote: ... the Csm complex was further purified on MonoS 4.6/100 PE (GE Healthcare) in 20 mM MES ...
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Impact of DNA sequences in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complexbioRxiv - Biochemistry 2020Quote: ... The complex was then purified by anion-exchange chromatography (Mono Q, GE healthcare) over a 0–2 M NaCl gradient in 5 mM BTP-HCl and 10% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... The MRPP1/MRPP2 complex was purified using a nickel affinity column (GE Healthcare) and the His-tag was removed from MRPP1 by by incubation with TEV protease ...
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bioRxiv - Biochemistry 2019Quote: ... The complex was further purified by HiTrap Heparin HP affinity column (GE Healthcare) after diluting eluant ~5-fold with 20 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was further purified using a MonoQ ion exchange column (GE Healthcare) equilibrated with MBP-binding buffer ...
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bioRxiv - Biophysics 2024Quote: ... the complex was purified by a Superdex 200 10/300 column (GE Healthcare) equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Molecular Biology 2023Quote: ... Z-stack images covering the entire thickness of the samples were acquired on a Delta Vision RT (Applied Precision) restoration microscope using a 100X/1.40 Plan Apo objective ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and ion-exchange (Hi-Load Q column, GE Healthcare) for the 5FW-labeled protein ...
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bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Immunology 2022Quote: ... Disulfide bond-linked soluble TCRs were produced as previously described.11 The binding between the soluble TCR and pMHC was determined using surface plasmon resonance (SPR) on a Biacore T200 (GE Healthcare) as previously described.11
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Genetics 2019Quote: ... All GST-tagged proteins were purified using glutathione beads (GE Healthcare) according to the protocol supplied by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... The GST-tagged constructs were purified with glutathione beads (GE Healthcare), whereas ion metal affinity chromatography using Ni2+ beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-tagged protein was purified by Glutathione sepharose (GE Healthcare), followed by on-column TEV cleavage ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fluorescence microscopy images of SSHELs were obtained using Delta Vision Core microscope with a Photometrics CoolSnap HQ2 camera (Applied Precision) as described previously 56 ...
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bioRxiv - Cell Biology 2020Quote: All yeast live-cell epifluorescent microscopy was performed on a Nikon Eclipse E600 or a Delta Vision microscope (Applied Precision) equipped with a Coolsnap HQ camera (Photometrics) ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired as 3D stacks using a 100X lens in a Delta Vision Deconvolution system equipped with an Olympus 1×70 microscope and images were deconvolved using SoftWoRx 3.0 (Applied Precision) and mounted using Photoshop.
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bioRxiv - Biochemistry 2022Quote: ... and the complex purified by affinity chromatography using the chelating Ni2+ Sepharose (GE Healthcare). After His-tag cleavage with the TEV protease ...
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bioRxiv - Microbiology 2020Quote: ... the complex was applied onto a HiLoad 16/600 Superdex 200 column (GE Healthcare) in buffer P ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... The complex was separated from excess CirpA1 by SEC (S200 16/60 GE Healthcare) in 20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The eluted LptDE complex was subsequently applied to a Resource-Q column (GE Healthcare), and followed by a Superdex™ 200 10/300 size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The complex was further purified on an ion-exchange column (Source-15Q, GE Healthcare) with Tris-HCl buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and the complex was purified over Hiload 16/600 Superdex 75pg column (GE Healthcare) pre-equilibrated with buffer containing 25mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein complex was further purified over a Ni-charged chelating column (GE Healthcare) and eluted by a linear gradient to 500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Molecular Biology 2020Quote: ... Loaded molecules were further purified by size exclusion using a Superdex 200 Increase 10/300 column (GE Healthcare) in High Salt SEC Buffer (1M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... followed by further separation from small molecules by passage through a pre-packed PD-10 column (GE Healthcare). Protein concentrations were determined by absorbance at 280 nm using a calculated molar extinction coefficient (ε280nm ...
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Bioengineering 2021Quote: ... PEGylated mEGFP tagged ferritin was purified with PD10 desalting columns (GE Healthcare) equilibrated with 20mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...