Labshake search
Citations for GE Life Sciences :
101 - 150 of 925 citations for Recombinant Human CD3E & CD3D His & Flag tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Flag immunoprecipitates of IGF-1R-Flag were incubated with Protein G Sepharose beads (GE Healthcare). Beads were pelleted ...
-
bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
-
bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
-
bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
-
bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and ion-exchange (Hi-Load Q column, GE Healthcare) for the 5FW-labeled protein ...
-
bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
-
bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
-
bioRxiv - Microbiology 2020Quote: ... The six-His-tagged BclA3CTD protein was purified under native conditions using the His-Trap column as recommended by the manufacturer (GE Healthcare Life Science). The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore) ...
-
bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
-
bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
-
bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
-
bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
-
bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
-
bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: His-tagged HexaPro was immobilized to a NiNTA sensorchip (GE Healthcare) to a level of ∼500 response units (RUs ...
-
bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
-
bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
-
bioRxiv - Molecular Biology 2019Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE healthcare). Histones were eluted by a salt gradient from 200 mM to 600 mM NaCl ...
-
bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
-
bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
-
bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
-
bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
-
bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
-
bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
-
bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
-
bioRxiv - Biophysics 2020Quote: ... The 6xHis-Lipo tag was cleaved off using Thrombin (GE Healthcare) and the protein was separated from the cleaved tag using the same column and buffers as described above ...
-
bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by Step-tag affinity chromatography using StepTactin resin (GE Healthcare) in BRB20 ...
-
bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
-
bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was fractionated using a Hi-Trap Ni2+ column (GE Healthcare) following the manufacturer’s instructions and checked by SDS-PAGE electrophoresis ...
-
bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
-
bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
-
bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...