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Citations for GE Life Sciences :
101 - 150 of 968 citations for Recombinant Human CD274 His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Biotinylated proteins were captured with streptavidin-sepharose (GE Healthcare) and then analyzed by western blotting.
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bioRxiv - Physiology 2021Quote: ... followed by biotinylated antibody (GE Healthcare, RPN1004V, 1:200), HRP-labeled streptavidin (Southern Biotech ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tagged TMIGD1 constructs in pGEX-4T-1 (GE Healthcare): GST-TMIGD1 (hTMIGD1 cytoplasmic tail (AA 242-262) ...
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bioRxiv - Developmental Biology 2019Quote: GST-tagged protein and bounded by Glutathione beads (GE healthcare). 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3) ...
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and ion-exchange (Hi-Load Q column, GE Healthcare) for the 5FW-labeled protein ...
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bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Systems Biology 2020Quote: ... biotinylated proteins were probed using HRP-conjugated Streptavidin (GE Healthcare Life Science ...
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bioRxiv - Neuroscience 2022Quote: ... streptavidin-biotinylated/HRP complex and ECL were from GE Healthcare. The HRP-labeled secondary antibodies used for western blot were from DAKO ...
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bioRxiv - Cell Biology 2023Quote: ... Marker lanes were incubated with Streptavidin-biotinylated-HRP (GE Healthcare). Proteins were visualized using chemiluminescent detection with ECL Prime Western Blotting Detection Reagents (Amersham RPN2232).
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were captured with Streptavidin Sepharose beads (GE Healthcare) and bound proteins analyzed by mass spectrometry.
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Genetics 2019Quote: ... All GST-tagged proteins were purified using glutathione beads (GE Healthcare) according to the protocol supplied by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... The GST-tagged constructs were purified with glutathione beads (GE Healthcare), whereas ion metal affinity chromatography using Ni2+ beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-tagged protein was purified by Glutathione sepharose (GE Healthcare), followed by on-column TEV cleavage ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Cell Biology 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Biochemistry 2019Quote: ... Biotinylated BET bromodomains were immobilised on a CAP chip (GE Healthcare) with a target density of ∼1000-1500 RU ...
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bioRxiv - Bioengineering 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Biochemistry 2022Quote: ... and biotinylated proteins were isolated using streptavidin-sepharose beads (GE Healthcare). Proteins were washed in ammonium bicarbonate and digested with trypsin ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Affimers were immobilized on a Sensor Chip SA (GE Healthcare) through streptavidin-biotin interaction ...