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Citations for GE Life Sciences :
251 - 300 of 1208 citations for Recombinant Human CASK His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and His-trap column (GE healthcare) previously equilibrated with Lysis Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare) by rotating in binding buffer (50 mM Tris HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... PreScission protease was removed by binding to GST-Sepharose (GE Healthcare) for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared lysates were loaded onto GST-Trap (GE Healthcare Life Sciences) washed with 20-30 column volumes wash buffer (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Biochemistry 2021Quote: ... GST fusion proteins were purified using Glutathione Sepharose HP (GE Healthcare) followed by cleavage with recombinant GST-HRV 3C protease and a GST recapture step ...
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bioRxiv - Molecular Biology 2022Quote: ... and GST (glutathione S-transferase) Sepharose beads (17075601, GE Life Sciences). Recombinant human HBP (2200-SE-050 ...
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bioRxiv - Neuroscience 2020Quote: ... GST protein was purified by adding Sepharose 4B beads (GE Healthcare) and incubating 2h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... GST-BirA was removed afterwards with Glutathione Sepharose 4B (GE Healthcare) resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... GST fusion proteins were affinity-purified on glutathione-Sepharose (GE Healthcare). For in vitro pull-down assays ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2020Quote: GST fusion proteins were cloned into pGEX6P1 (GE Healthcare, Pittsburgh, PA). MBP fusion proteins were cloned into pJP1.09 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lysate was subjected to GST-affinity chromatography (GE Life Sciences), the GST tag was removed by PreScission protease ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-GST HRP-conjugated antibody (RRID:AB_771429; GE Healthcare; 1:10,000). Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... using a His SpinTrap column (GE Healthcare). Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Microbiology 2023Quote: ... Anti-His antibody was from GE Healthcare; anti-PhoP antibody was elicited in rabbit (AlphaOmegaSciences) ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... except that the column was connected to a GST-Trap (GE Healthcare) for online removal of GST and the GST-3C protease.
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were coupled to glutathione sepharose (GE Healthcare, Freiburg, Germany) using 1x buffer B (10 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... goat polyclonal anti-GST (GE healthcare, 27-4577-01; 1:2000, IB); rabbit polyclonal anti-Cap1 [1 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... GST-fused proteins were purified using glutathione 9epharose 4B beads (GE HealthCare) equilibrated in 1x Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 4-mL GST column (GE Healthcare) equilibrated in PBS containing 1 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with 250 μL of GST Sepharose 4B (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... GST-fusion proteins were purified using glutathione-Sepharose 4B columns (GE Healthcare). Protein-bound glutathione beads were thoroughly washed with cold PBS six times ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was purified on GST Sepharose 4B resin (GE healthcare) and then eluted in the lysis buffer with 25mM glutathione ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was incubated with GST beads (Glutathione Sepharose 4B, GE Healthcare) for 2 hours at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... Affinity purification of GST-SseK3 was performed using glutathione sepharose (GE Healthcare), and purification of 6×His-SUMO-hUBE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... GST fusion proteins were affinity purified over Gluthione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...