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Citations for GE Life Sciences :
201 - 250 of 806 citations for Recombinant Human AKT3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... GST-fusion proteins were purified using glutathione-Sepharose 4B columns (GE Healthcare). Protein-bound glutathione beads were thoroughly washed with cold PBS six times ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was purified on GST Sepharose 4B resin (GE healthcare) and then eluted in the lysis buffer with 25mM glutathione ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was incubated with GST beads (Glutathione Sepharose 4B, GE Healthcare) for 2 hours at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... Affinity purification of GST-SseK3 was performed using glutathione sepharose (GE Healthcare), and purification of 6×His-SUMO-hUBE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... GST fusion proteins were affinity purified over Gluthione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins were pull-down with glutathione Sepharose 4B beads (GE Healthcare), pre-washed with ice-cold PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Glutathione sepharose 4B GST beads was purchased from GE Healthcare (Mississauga, ON, Canada). Lipofectamine 2000 and Alexa Fluor 488 goat anti-mouse IgG were purchased from Invitrogen (Burlington ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The GST fusion proteins were purified with Glutathione Sepharose ™4B (GE Healthcare) according to the manufacturer’s protocol with Binding Buffer I [50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibodies were directed against GST (GE Healthcare Cat# 27-4577-01, RRID:AB_771432), murine AOC3 (Abcam Cat# ab42885 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell lysates were precleared with GST immobilized on glutathione–Sepharose 4B (GE Healthcare) and then incubated with the indicated GST fusion proteins (2–5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... GST-β2HA was purified from the soluble fraction using glutathione resin (GE Healthcare). The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Biophysics 2023Quote: ... The clarified supernatants were loaded onto a GST column (GSTrap HP, GE Healthcare) or a nickel affinity chromatography column (HiTrap HP ...
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bioRxiv - Plant Biology 2024Quote: ... GST-TZF1 was purified using glutathione-Sepharose 4B resin (GE Healthcare Bio Sciences) as previously described (Qu et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... GST-OsbZIP86 and MBP-OsSAPK10 were incubated with Glutathione Sepharose 4B (GE Healthcare, USA) in pull-down buffer (50 mM Tris-HCl ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and GST-hFMRP KH0-KH1-KH2 were purified using glutathione affinity chromatography (GE Healthcare). All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The GST-CP was bound to glutathione Sepharose beads (GE Healthcare, 17- 5132-01) for 3 h at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare) for 1 hour at 4 °C with gentle rocking ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... cell lysates of these complexes were firstly treated with GST agarose beads (GE Healthcare). And then the protein-bound beads were transferred into gravity columns and treated with 3C protease overnight at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Cell Biology 2019Quote: ... The chip was prepared by covalent coupling of an anti-GST antibody (GE Healthcare) as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... and the GST-WDR41 fusion was generated in pGEX-6T-1 (GE Life Sciences). These new plasmids were generated by Gibson Assembly or Q5 Site-Directed Mutagenesis according to manufacturer instructions (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... GST-TLK2 were pulled down by Glutathione Sepharose 4B (GE Healthcare #17-0756-01) for 1 hour rotation at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
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bioRxiv - Biochemistry 2023Quote: USP7 and GST: SPR experiments were performed on a Biacore T200 instrument (GE Healthcare) at 25 °C using a running buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was removed using PreScission protease (GE Healthcare Life Sciences (now Cytiva)) ...
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bioRxiv - Plant Biology 2023Quote: ... The soluble fractions were collected and allowed to flow through GST resin (GE Healthcare). After washing with two column volumes of the same buffer used for resuspension ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... The GST-ScLas1 mutants were further purified on a Heparin HP column (GE Healthcare) and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...