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Citations for GE Life Sciences :
101 - 150 of 806 citations for Recombinant Human ACP1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The bait protein GST-WL1 was incubated with GST beads (GE Healthcare, Chicago, IL, USA) in non-EDTA buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-AtEPL1A and GST-AtEPL1B were purified on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... GST-TRAF6RING and GST-RNF38RING (residues 387-465) were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Microbiology 2020Quote: ... GST-E1 and GST-PknG were removed from the reactions with glutathione-Sepharose 4B (GE Healthcare). Next ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Developmental Biology 2020Quote: ... or GST Sepharose beads (GE Healthcare). GST pull-down was performed as described previously (Wei et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL GST beads (GE Healthcare) were added to the system for 2 h on a rotor at 4 °C.
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bioRxiv - Cell Biology 2022Quote: ... anti-GST (GE Healthcare, Cat# 27457701), anti-HA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... GST (GE Healthcare, 27-4577-01), and secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... or GST agarose resin (GE Healthcare), and purified via size-exclusion chromatography using a Superdex 200 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: GST-Byr2 (65-180) and GST-Scd1 (760-872) were purified using glutathione sepharose 4B (GE Healthcare). The proteins bound on the glutathione beads were washed with buffer A and used for the GST pull-down assays together with the Ras1.G17V (1-172 ...
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bioRxiv - Microbiology 2023Quote: ... The fusion construct GST SifAWT or GST SifAK11R were purified by using glutathione sepharose beads (GE Healthcare) and further cleaved with thrombin for tag separation ...
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bioRxiv - Molecular Biology 2023Quote: ... and GST-G3BP1-ΔIDR3 (deleting E411-Q466) was then loaded on a GST HP column (GE Healthcare) equilibrated with GSTBufferA (50 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... Purified GST-Slp1(456-488aa) was detected with either goat anti-GST HRP-conjugated antibodies (GE Healthcare) or rabbit polyclonal anti-pThr480 antibodies.
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Cdc11 and GST alone were immobilized on 100 µl equilibrated Glutathione sepharose slurry (GE Healthcare, Freiburg, Germany) directly from the crude extract ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GST (GE Healthcare, 27-4577-01); anti-G9a (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... once loaded GST-PreScission Protease (GE Healthcare) was injected into the column and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST (27-4577-01; GE Healthcare). Secondary antibodies were purchased from Li-cor Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... The pGEX-4T-1 (GST) (GE Healthcare) and pMAL-c5x (MBP ...
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bioRxiv - Cancer Biology 2021Quote: ... KIX domain was cleaved from GST by incubation of resin-bound GST-KIX with 160 U thrombin (GE Healthcare) overnight at room temperature ...
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
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bioRxiv - Neuroscience 2021Quote: ... GST (goat, GE Healthcare, 27457701V, WB 1:5000), MYC (mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were bound to GST resin (GE Healthcare) and eluted in buffer A supplemented with 10 mM glutathione (pH-calibrated) ...
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bioRxiv - Plant Biology 2022Quote: ... and GST-fusions by glutathione-resin (GE Healthcare), each according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with GST-CDK2 and then purified by affinity chromatography using Sepharose 4B GST resin beads (GE Healthcare) and subsequently eluted in 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: 10 μM purified WIPI2d was mixed with 20 μM of GST or GST-ATG16L1(207-230) and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 25mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... 80 picomoles of GST or GST-Tvactin immobilized on 20 μl of glutathione conjugated-sepharose beads 4B (GE healthcare, Illinois, USA) was incubated with 80 picomoles of His-TvFACPα or derived mutants in 1 ml GST binding buffer (PBS contains 0.2% Triton X-100 and 1 mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts of GST or GST-fusion proteins were incubated for 0.5-1h with glutathione-coated sepharose beads (GE Healthcare, Freiburg, Germany) equilibrated in PBS (Boi1-Bni1 ...
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bioRxiv - Microbiology 2021Quote: Binding of C1 and its sub-components to GST-ErpB and GST-ErpQ was performed at 25°C using a Biacore T200 (GE Healthcare) as previously described (Garcia 2016) ...
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bioRxiv - Biochemistry 2020Quote: ... purified fluorescent ULK1 complex (FIP200-MBP/EGFP-ATG13/EGFP-ATG101/ULK1) was mixed with purified GST-NDP52 or GST-4xUb and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... 500μl of dialyzed GST-Bsd or GST was bound to 50μl of PBS washed glutathione sepharose beads (GE Healthcare, 17-0756-01) and incubated at 4°C for 30min ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... and GST were purified on Glutathione Sepharose (GE Healthcare) by batch purification ...