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Citations for GE Life Sciences :
251 - 300 of 4139 citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...
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bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with protein A or protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 3 h with gentle rocking in the cold room ...
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Large scale protein purification was done using ÄKTA prime Fast Liquid Protein Chromatography System (GE Healthcare). DE droplets were analyzed on a BD LSR Fortessa SORP (Special Order Research Product ...
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bioRxiv - Immunology 2021Quote: ... and the protein within the supernatant was purified with a Protein A-Sepharose column (GE Healthcare) according to the manufacturer’s instruction for primary purification ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Microbiology 2022Quote: ... and isolated from supernatants by affinity chromatography using Protein A or Protein G columns (GE Healthcare). MAbs were eluted with 0.1 M glycine at low pH and dialyzed into PBS.
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Protein G sepharose beads (GE Healthcare) were washed three times in lysis buffer to remove any residual ethanol from the storage buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G Sepharose (GE Healthcare) pre-blocked in 1% BSA-PBS were used to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Molecular Biology 2021Quote: ... A Protein-G column (GE Healthcare) was used for affinity purification of antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G-agarose beads (GE Healthcare) were added to the lysate–antibody mix and left to rock at 4°C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... RDR2 and protein standards (GE Healthcare) were subjected to FPLC using a Superdex 200 10/300 GL column at 4°C using 50 mM HEPES-KOH pH7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... protein G Sepharose beads (GE Healthcare) were blocked overnight with 0.5 mg BSA ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare) was added to Expi293 supernatant for 2h at room temperature or overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Protein A sepharose beads (GE Healthcare) were washed twice and resuspended 1:1 with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... and Protein G sepharose (GE Healthcare) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G-Sepharose beads (GE Healthcare) were added to the supernatant and incubated at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-G Sepharose beads (GE Healthcare) were blocked in 0.1% BSA for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G–agarose beads (GE Healthcare) or magnetic beads were washed twice before immunoprecipitation.
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bioRxiv - Genetics 2023Quote: ... and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... with protein A column (GE Healthcare). MARV GP (Uganda strain ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins and SP3 beads (GE healthcare) were mixed in a 1:10 weight ratio in 80 % ethanol for 5 minutes at RT ...
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bioRxiv - Biochemistry 2023Quote: ... KTA Protein Purification Systems (GE Healthcare) and UNICORN Control Software version 5.11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: Protein A-Sepharose beads (GE Healthcare) were incubated with 2 µg of antibody (anti-V5 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) and chemiluminescence imaging using LAS-4000 system (Fuji Film) ...
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bioRxiv - Microbiology 2022Quote: ... and goat anti-mouse or anti-rabbit HRP conjugates (GE Healthcare).
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bioRxiv - Systems Biology 2021Quote: ... primary antibodies and polyclonal goat anti-mouse HRP conjugated (GE Healthcare) secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Cy3-labelled goat-anti-mouse mAb (PA43009, GE Healthcare, 1:3,000) was subsequently used as a secondary antibody ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-goat as the secondary antibody (GE Healthcare) were used at a dilution of 1:3000.
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Genetics 2022Quote: ... and then incubated with goat-developed anti-rabbit (GE Healthcare, NA934) or goat-developed anti-mouse immunoglobulin G (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 000 dilution and HRP Goat anti-rabbit IgG (GE Healthcare, NA934) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-GST HRP-conjugated antibody (RRID:AB_771429; GE Healthcare; 1:10,000). Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant segment was purified by Ni2+-affinity chromatography using HisTrap HP purification columns with a FPLC system (ÄKTA Purifier, GE Healthcare) with elution buffer consisting of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were dialyzed into 20 mM sodium phosphate pH 7.4 with 500 mM sodium chloride and the recombinant RBD was purified by immobilized metal ion affinity chromatography (IMAC) using a HisTrap excel column (GE Healthcare). The purified protein was biotinylated using the E ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The protein-protein complex was then passed through a size-exclusion column (Superdex75, 26/60, GE Healthcare) to remove unbound proteins ...