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Citations for GE Life Sciences :
201 - 250 of 4136 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... coli and purified using a HisTrap FF Ni-NTA column (GE Healthcare Life Sciences). The SUMO tag was cleaved by incubation with SUMO protease (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... coli expressed SDAg6 to CNBr-Activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells from the pDYH24 plasmid and purified with glutathione sepharose (GE Healthcare). PUD-1/PUD-2 heterodimers were purified on a HisTrap column followed by gel filtration ...
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bioRxiv - Cell Biology 2023Quote: ... coli extracts on 100 μl Glutathione–Sepharose beads in PBS (GE Healthcare, Freiburg, Germany). After incubation for 1h at 4°C with either E.coli extracts or purified proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cell fluorescence was carried out using a Typhoon FLA 9500 fluorimager (GE Healthcare) as described in Olorunniji et al ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing RNase E were pooled and loaded on a butyl sepharose HP column (GE Healthcare) which previously was equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... coli BW25113 and the single-gene knockout mutants Δpgm were purchased from Dharmacon (GE Healthcare) and stored in 50% glycerol stock at -80°C ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 and affinity-purified with GST beads (#17-0756-01, GE Healthcare Life Sciences). 293T cells overexpressing indicated proteins were treated with DNA damage reagents and lysed ...
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bioRxiv - Biochemistry 2023Quote: ... coli was subsequently purified by cation-exchange chromatography with a HiTrap™ SP (GE Healthcare) column ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system (GE Healthcare). First ...
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bioRxiv - Microbiology 2019Quote: ... Purifications of the GST-tagged recombinants were performed as described by the manufacturer (GE Healthcare). For expression in mammalian cells ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cultures was detected using a UV/visible spectrophotometer (Ultrospec TM 2100 pro, GE Healthcare, UK). The quantification of L-lysine ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant APE1 were then further purified by Superdex 200 increase 10/300 chromatography (GE Healthcare) in the buffer containing 20 mM Tris-HCl (pH 7.0) ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... B-mode ultrasonography of the chest was performed (18;19) using a Logiq e system (GE Healthcare, Milwaukee, WI) using R5.2.x software and a multifrequency transducer model 12L-RS at a working frequency of 10 MHz ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... coli BL21(DE3) pLysS and purified from inclusion bodies via Sephacryl S-200 gel filtration (GE Healthcare). Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... Recombinant D9 was purified sequentially by a Ni-NTA column followed by a heparin column (GE Healthcare) and a GE Superdex 75 16/60 gel filtration column to separate aggregates and exchange into crystallization buffer (10 mM MES pH 5.6 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 and were purified by immunoprecipitation with glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare). Each reaction with 30 μl final volume contained 50 ng Wheat E1 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...