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Citations for GE Life Sciences :
351 - 400 of 1392 citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C overnight and then scanned using an Amersham Typhoon scanner (GE Healthcare). Individual infected cells were quantified using ImageQuant TL software (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... To isolate activated protein C (aPC) ion exchange chromatography with FPLC (ÄKTAFPLC®, GE Healthcare Life Sciences) was used ...
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bioRxiv - Plant Biology 2022Quote: ... Gels were dried at 80 °C for 2 hrs and covered with phosphor imaging plate (GE Healthcare) overnight after RNA fixation in RNA fixation buffer (40% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Soluble PB2 C-terminal domains were purified by affinity chromatography on HisTrap columns (GE Healthcare Life Sciences) and analyzed by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-fused IQGAP1-N and IQGAP1-C were purified using Glutathione Sepharose 4B (17-0756-01; GE Healthcare). Next ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... These were stored in a chilled compartment at 9°C within a skirted 384 well plate (GE Healthcare) before being washed over the chip surface in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: All SPR measurements were performed at 25° C using a Biacore 3000 instrument (BIAcore, GE Healthcare, Piscataway, NJ) as described previous24,25 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Immunology 2022Quote: The hcAb-binding properties of B10 were analyzed at 25 °C on a Biacore T200 instrument (GE Healthcare) using 10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then separated in 10% SDS polyacrylamide gels and transferred to Hybond C nitrocellulose membranes (GE Healthcare). After blocking with 5% non-fat milk in PBS/0.05% Tween ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Immunology 2021Quote: All surface plasmon resonance experiments were performed at 25°C using a Biacore T200 instrument (Cytiva, GE Healthcare) equipped with a flat carboxymethyldextran sensor chip (CMDP ...
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bioRxiv - Cell Biology 2021Quote: ... The experiments were performed at 25°C using a BiacoreTM T200 instrument (GE Healthcare Life Sciences, Uppsala, Sweden) flowed with EP+ buffer (10mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare). Proteins were exchanged into 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Live imaging was carried out in a chamber at 37°C using a Deltavision Core (Applied Precision™) microscope equipped with a CoolSnap HQ2 (Photometrics ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Biochemistry 2022Quote: Ca2+ binding was characterized by ITC at 25 °C using a VP-ITC microcalorimeter (GE Healthcare, Northampton, MA) with a cell volume of 1.4619 ml in Na+ or K+ containing buffers (20 mM Tris pH 7.9 ...
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bioRxiv - Microbiology 2023Quote: The surface plasmon resonance (SPR) experiments were performed at 25 °C on a BIAcore 2000 instrument (GE Healthcare). DsrC-trisulfide was immobilized on a NTA sensor chip (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...