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Citations for GE Life Sciences :
1 - 50 of 2332 citations for Rat Histone Deacetylase 1 HDAC1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligated full-length modified histone was separated from unligated histone through cation exchange chromatography on a HiTrap SP column (GE Healthcare) and then dialysed against three changes of water containing 5 mM β-mercaptoethanol before lyophilization and storage at −a80°C until use ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... each histone was subjected to Resource S column chromatography (GE Healthcare). The column was washed with buffer D (20 mM sodium acetate (pH 5.2) ...
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bioRxiv - Biophysics 2019Quote: ... The mutant histone was purified and labeled with Cy3 maleimide (GE Healthcare) under denaturing conditions (Harada et al. ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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bioRxiv - Molecular Biology 2021Quote: ... Histones were then purified further by passing over a HiTrap Q column (GE Healthcare) before binding to and NaCl gradient elution from HiTrap SP cation exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting histone H2A was further purified by HiTrap SP column chromatography (GE Healthcare), flash-frozen with LN2 ...
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bioRxiv - Biochemistry 2020Quote: ... Excess histones and enzymes were removed using a S-400 spin column (GE Healthcare). ATPase reactions were initiated by adding an ATP/MgCl2 Mix (final concentration 2.5 mM each) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAi efficiency testing for ELAV was done from dissected central brains by Western blotting according to standard protocols as described 73 using a polyclonal rat anti-ELAV antibody generated against Drosophila ELAV (1:800) 45 and secondary HRP-coupled goat anti-rat antibody (1:5000, GE Healthcare) by chemoluminescence detection (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... The radiolabelled histone H1 was then detected using a BAS Storage Phosphor screen (GE Healthcare) and read out using a Phosphorimager Typhoon 8600 (Molecular Devices).
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat IgG-HRP (GE Healthcare, UK), anti-rat IgG-HRP (Cell signaling ...
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bioRxiv - Biochemistry 2020Quote: ... Histone octamers were purified by size exclusion chromatography (HILoad 16/600 Superdex 200 column, GE Healthcare) and stored at −20 °C in 50 % glycerol.
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bioRxiv - Biophysics 2020Quote: ... The histone octamers were purified by size-exclusion chromatography on a Superdex 200 column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Histone octamers were purified by size exclusion chromatography (HILoad 16/600 Superdex 200 column, GE Healthcare) and stored at −20 °C in 50 % glycerol.
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... histones were purified on a gel filtration column HiLoad Superdex 75 pg (GE Healthcare Life Sciences) pre-equilibrated with histone gel-filtration buffer (HGFB ...
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bioRxiv - Molecular Biology 2023Quote: ... Histone octamer was purified by Superdex 200 Increase 10/300 GL column-based SEC (GE Healthcare) that was pre-equilibrated with refolding buffer ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Biophysics 2020Quote: ... equimolar amounts of all histones were dialyzed and the octamer was purified over Superdex 200 (GE healthcare). The DNA and octamer was mixed in a 1:1.1 ratio and purified over a BioRad prep cell after overnight gradient salt dialysis ...
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bioRxiv - Biochemistry 2021Quote: ... the histone octamer solution was purified via gel filtration (Superdex 200 Increase 10/300 GL; GE Healthcare) that had been pre-equilibrated with Octamer Refolding Buffer ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The different histone chaperone constructs were purified by gravity-flow chromatography using a Ni-NTA resin (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated histone octamers were further purified on a 120 ml Superdex 200 size exclusion column (GE Healthcare) with SEC buffer (30 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... mouse or rat IgG was purchased from GE Healthcare.
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bioRxiv - Molecular Biology 2021Quote: ... correctly assembled histone octamers were purified by size exclusion chromatography in refolding buffer on a S200 column (GE Healthcare), using an Akta PURE system (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... and RbmAb-phospho-histone H3 (Ser10/Thr11) were detected with donkey anti-rabbit IgG conjugated to HRP (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... the histones were purified from inclusion bodies under denaturing conditions on a 5 mL HiTrap SP FF (GE Healthcare) cation exchange column on a Next Generation Chromatograph (NGC ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-linked goat anti-rat IgG pAb (#NA935V, GE Healthcare) and HRP-linked rabbit anti-goat IgG pAb (DAKO #P0449 ...
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bioRxiv - Biophysics 2022Quote: ... Histones were extracted from inclusion bodies under denaturing conditions and purified through Q FF and SP FF columns (GE Healthcare). Histone octamers were then refolded by dialysis and purified by gel filtration using a Superdex 200 10/300 GL column.
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bioRxiv - Biochemistry 2020Quote: ... The octamer of tailless histones was purified by size-exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare), which separated the octamer from aggregate ...
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl) and the resulting histone octamer purified using a Superdex 200 gel filtration column (GE Healthcare). Purified 147bp DNA carrying the 601 nucleosome-positioning sequence was a kind gift from the Brockdorff lab ...
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bioRxiv - Systems Biology 2021Quote: ... Phosphorylated histone bands were visualized by autoradiography and analysed using a phosphoimager (Storm 860, GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The extent of phosphorylation was quantified as described above.
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bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
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bioRxiv - Biophysics 2022Quote: ... ELISA was performed as described2 using an HRP-conjugated anti-M13 monoclonal antibody (GE Healthcare) and a TMB substrate kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).