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Citations for GE Life Sciences :
401 - 450 of 6114 citations for Rat Heat Shock Factor Protein 1 HSF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified by affinity chromatography with nickel affinity columns (HisTrap 1 ml; Cytiva) using the Äkta pure system (GE Healthcare UK Ltd.). Elution was carried out at a flow rate of 1 ml/min with all solutions maintained at 4 °C and a linear imidazole concentration gradient from 30 mM to 530 mM.
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Microbiology 2022Quote: ... High performance liquid chromatography (HPLC) was conducted using a HiTrap Q XL 1 mL Sepharose column on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). Proteins were eluted with starting buffer A followed by a gradually increasing concentration of buffer B (20 mM Bis-Tris ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA were extracted using the Illustra RNAspin kit and treated with DNase 1 (GE Healthcare). cDNA synthesis and taqman qPCR were performed as previously described [16] ...
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bioRxiv - Zoology 2021Quote: ... One of the obtained PCR products was purified with ExoProStar 1-Step kit (GE Healthcare US77702) and Sanger-sequenced directly at Macrogen (Netherlands) ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were exposed with ECL prime (GE Healthcare) at a ChemiDox XRS imaging system (Bio-Rad).Primary antibodies were used against H3K27me3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare) and detection was performed using anti-His primary antibody (Penta-His Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250 µl slurry ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G–Sepharose (GE Healthcare, 17-0618-01). Dynabeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... using a protein purification system (ÄKTApurifier, GE Healthcare). Elution of the proteins was achieved by using a linear gradient of imidazole from 4 mM to 500 mM with sodium phosphate buffer B (20 mM Na2HPO4 x 2H2O ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified on protein A column (GE healthcare). Proteins were buffer exchanged and concentrated in PBS pH7.4.
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bioRxiv - Biophysics 2020Quote: ... The protein A sensor chips (GE Healthcare Lifesciences) were loaded by injecting each ligand for 100 s until to reach a signal level between 100 and 120 arbitrary response units (R.U. ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µl of Protein A agarose (GE Healthcare) was coupled with 2 µg of purified antibodies or 5 µl of antiserum ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto nitrocellulose membrane (GE Healthcare) by wet blotting overnight at 8°C and 27 V ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were revealed with ECL Prime (GE Healthcare) and images were acquired using a LAS4000 Camera (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), then blocked with 5% FBS or 5% milk in TBST and probed for 2 h with primary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were electroblotted onto PVDF membrane (GE Healthcare), blocked with 5% nonfat milk in TBS with 0.1% Tween-20 for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μl Protein G-Sepharose matrix (GE Healthcare) was added which was previously equilibrated in TBS buffer and this mixture was further incubated for another 2 h on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) and blocked for 1 hour with Odyssey Blocking Buffer (Li-COR bioscience ...
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bioRxiv - Biochemistry 2020Quote: Protein A-Sepharose CL-4B beads (GE Healthcare) were prepared by first washing them twice in IPP150 buffer containing 20 mM Tris-base ...
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bioRxiv - Neuroscience 2019Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250µl slurry ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein was transferred to nitrocellulose membrane (GE Healthcare). Membrane was blocked in 5% milk in TBST (TBS + 0.1% Tween-20) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using ECL reagents (GE healthcare) in a Biorad ChemiDoc XRS+ ...