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Citations for GE Life Sciences :
351 - 400 of 1006 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Then the peak fractions were desalted and further purified by ion-exchange chromatography using 1-ml Resource S column (GE Healthcare) in buffer E (20 mM mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: For crystallization trials the ATAD2 BRD was further purified by gel-filtration using a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare). The purified ATAD2 BRD 966-1112 ...
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bioRxiv - Pathology 2021Quote: ... and 3500 V for 28 kVh (linear) with a Hoefer™ IEF 100 (M/s. GE Healthcare Life Sciences, Amersham, UK) at 20 °C ...
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bioRxiv - Immunology 2020Quote: ... Purified mAbs were first immobilized at coupling densities of 800-1200 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% sodium azide buffer ...
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Microbiology 2021Quote: Purified TlpALBD was immobilized on a CM5 series S sensor chip and binding affinities tested using a BIAcoreTM S200 instrument (GE Healthcare) as described (Day et al. ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Cell Biology 2022Quote: ... epifluorescence microscopy as well as time-lapse experiments (500 ms time-lapse for 60 s) were performed on a Deltavision OMX V4 microscope (GE Healthcare) equipped with three water-cooled PCO edges CMOS cameras ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 10 mL 1X S basal buffer for COPAS biosorter analysis and imaging on a GE InCell analyzer 2200 (GE Healthcare). See Supplementary Methods for full details.
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bioRxiv - Molecular Biology 2022Quote: ... The purified MUS81-EME1 was subjected to cation-exchange chromatography (CEC) with a Mono S 4.6/100 PE column (GE Life Sciences) on ÄKTA explorer FPLC system ...
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bioRxiv - Immunology 2023Quote: SPR measurements were performed using a Biacore T200 (GE Health-care) instrument docked with an S CM5 series sensor chip (GE Healthcare), as described (Murugan et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Cat#47410-26615 or Cat#47410-07595) or with a FUSION Solo S imaging system (VILBER) using ECL reagents (GE Healthcare). Quantification was performed via densitometry using ImageJ40 ...
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bioRxiv - Biophysics 2023Quote: VD1 hydrodynamic properties were approximated by SEC using a HiLoad 16/60 Superdex 75 prep grade (S-75) column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Biophysics 2023Quote: ... the remaining sample was centrifuged at 16,000 x g for 10 min to remove any precipitate and loaded onto a HiPrep 16/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) pre-equilibrated in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Biochemistry 2022Quote: ... The ACE2-induced postfusion S-nanodisc sample was purified by gel filtration chromatography on Superose 6 Increase 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Genomics 2023Quote: ... Monosomes were isolated with size exclusion chromatography using the Illustra MicroSpin S-400 HR Columns (GE life sciences, cat. no. 27514001) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: SPR measurements were performed using a Biacore T200 (GE Health-care) instrument docked with Series S Sensor Chip CM5 (GE Healthcare), as previously described36 ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
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bioRxiv - Genomics 2023Quote: ... and the lysate were passed through a size exclusion column (Illustra MicroSpin S-400 HR Columns; GE Healthcare, 27-5140-01). RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... of SOD1 is further purified by size-exclusion chromatography (SEC) using a custom made gel filtration column (dimensions: id 1.2×100cm) packed with SephacrylTM S-200 (GE Healthcare, USA) in order to to isolate the dimeric protein fraction in TBS buffer ...
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bioRxiv - Biochemistry 2023Quote: ... monomeric actin was separated from oligomeric species by passing it through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) equilibrated in G-buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare). The purified CapZ was flash-frozen in liquid N2 and stored at −80 °C in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The refolded WT- and E111V-GCAP1 variants were purified by size exclusion chromatography (SEC, HiPrep 26/60 Sephacryl S-200 HR, GE Healthcare) followed by anion exchange chromatography (AEC ...
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bioRxiv - Cell Biology 2024Quote: ... We pooled the α-syn-containing fractions and further purified the protein by size-exclusion chromatography on a HiPrep Sephacryl S-200 HR 16/60 gel filtration column (GE Healthcare) in 50 mM NH4Ac pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat. # 17-0476-01, GE Healthcare). Second ...
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bioRxiv - Synthetic Biology 2022Quote: ... a size exclusion purification step was performed using a S500 column HiPrep Sephacryl 16/60 S-500 HR (GE Healthcare Life Sciences), equilibrated with PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... A portion (20.9 mg) of this LPS preparation was further purified by gel permeation chromatography on Sephacryl S-400 HR (GE Healthcare, Uppsala, Sweden) on a column (2.5 × 120 cm ...
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bioRxiv - Molecular Biology 2023Quote: ... The precipitate was then resuspended in BD buffer (BC buffer with 50mM Ammonium sulphate) with 500mM KCl and fractionated by molecular weight on the gel filtration column Sephacryl S-400 (GE healthcare, USA) with a fractionation range of 2×104–8×106 Da ...
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bioRxiv - Cancer Biology 2023Quote: ... Imaging of membranes for chemiluminescence or after ponceau S staining was performed using the Amersham Imager 600 (GE HealthCare Life Sciences, USA). Quantification of protein expression was performed using ImageJ software ...
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bioRxiv - Developmental Biology 2023Quote: ... Chemiluminescent substrate was added according to the manufacturer’s instructions (Amersham ECL Prime Western Blotting Detection Reagent, Cytiva #RPN2232) and was detected with an Amersham Imager 600 (GE Healthcare, 10600023). The antibodies used in this study are listed in Table S16.
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bioRxiv - Molecular Biology 2024Quote: The SPR experiments were performed using a Biacore T200 equipped with a Series S Sensor Chip SA (GE Healthcare #BR-1005-31). Biotinylated MproQ306E.AVI (35752 Da ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...