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Citations for GE Life Sciences :
601 - 640 of 640 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... gel filtration column with a flow rate of 1 ml/min using FPLC systems (AKTA pure 25 and AKTA FPLC, GE Healthcare Life Science). The 5 ml sample concentrated ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and then incubated with 500-μl of Nickel-Sepharose 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the crosslinked-sample was again separated with a flow rate of 0.5 ml/min on a Superdex 200 10/300 GL column (GE Healthcare, Little Chalfont, UK), previously equilibrated with buffer S.
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.
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bioRxiv - Microbiology 2019Quote: ... SepFΔML,K125E/F131A were covalently immobilized on 3 independent flow-cells of an NTA sensorchips as previously described 64 (GE Healthcare Life Sciences). The final immobilization densities of SepF variants ranged from 3000 to 4000 resonance units (RU ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2020Quote: ... The lysate was then subjected to nickel-immobilized metal affinity chromatography (IMAC) using a HisPrep™ Fast Flow 16/10 column (GE Healthcare, USA) equilibrated with equilibration buffer (20 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... by injecting the sample onto a 24 x 1 cm size exclusion column containing Sepharose 4B Fast Flow resin (pore size 84 nm) set-up in an AKTA Start System (GE Healthcare Life Science). EVs were eluted in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The CM was then concentrated by tangential-flow filtration with a 100 kDa molecular weight cut-off filter membrane cartridge (GE Healthcare, Chicago, USA) and buffer exchange was performed by diafiltration with EDB1 (Dilution Buffer with 2%Trehalose in PBS) ...
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bioRxiv - Biophysics 2024Quote: ... The flow-through was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL size exclusion column GE Healthcare Life Sciences) in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
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bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was subjected to serial purification steps using Superdex 200 Increase 10/300 G size-exclusion and Hitrap Q Sepharose Fast Flow anion-exchange columns (GE Healthcare, Pittsburgh, PA, USA). Purified a-syn was applied to High capacity endotoxin removal spin columns (Pierce ...
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bioRxiv - Immunology 2022Quote: ... The flow-through was collected and concentrated before injection onto a HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare Diegem, Belgium) using HBS as running buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... The flow-through was collected and purified by ion-exchange chromatography on a HiTrap Q HP column (GE Healthcare Life Sciences, Chicago, IL, USA), using Buffer I1 (20 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... or 25 °C (ASK1 TBD-CRR and ASK1 CRR) for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Biophysics 2021Quote: ... on a gravity flow column followed by size-exclusion chromatography on a Superdex® 200 Increase 10/300 GL column (GE Healthcare Life Sciences, USA) in a buffer containing 10 mM NaCl and 10 mM sodium phosphate ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto a nickel (II) 5 mL HisTrap packed with Sepharose® 6 fast flow agarose beads (GE Healthcare Biosciences, NSW, Australia) using an ÄKTApure™ system (GE Healthcare Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were then eluted with 100 mM L-glutathione reduced and subjected to a second purification by addition of Nickel-Sepharose beads 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA). The protein was eluted with HKI500 buffer (20 mM Hepes ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... using a series of chromatography steps (XK 50/30 cationic exchange column [GE Healthcare] packed with SP-Sepharose Fast Flow [GE Healthcare], MonoQ HR 16/10 anionic exchange column [GE Healthcare] and TSKgel G4000SWxl column). SNAP-BICD2N and BICD2N-GFP (both containing residues 1-400 of mouse BICD2 ...