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Citations for GE Life Sciences :
101 - 150 of 387 citations for RFamide Related Peptide 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Biophysics 2021Quote: ... the FLAG-tagged Ubr1 was eluted with 1mg/mL FLAG peptide and further purified by a Superose 6 size-exclusion column (GE Healthcare) equilibrated in a buffer captaining 50 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... peptides were further fractionated prior to LC-MS by reversed-phase HPLC using an Ettan ÄKTA micro HPLC system (GE Healthcare), as described elsewhere98.
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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bioRxiv - Biophysics 2019Quote: ... The dissolved peptide was then fractionated on a Superdex-75 HR 10/30 size exclusion chromatography (SEC) column (GE Life Sciences) on a BIORAD FPLC system that was pre-equilibrated with 20mM Tris at pH 8.00 ...
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bioRxiv - Biochemistry 2021Quote: ... The separation of unreacted [35S]-fMet and each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Microbiology 2021Quote: ... The separation of unreacted [35S]-fMet from each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Microbiology 2019Quote: ... TolA was loaded into the cell and synthetic TolB peptide (residues 22-33; 2 mM) loaded into the syringe of an iTC200 microcalorimeter (Microcal/GE Healthcare). Titration consisted of 20 injections (1 x 0.4 μl ...
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bioRxiv - Biochemistry 2021Quote: SPR experiments were carried out to characterize the interaction between PKAc and substrate peptides using a Biacore T200 instrument (GE Healthcare). PKAc was immobilized via standard N-hydroxysuccinimide (NHS ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Biophysics 2022Quote: ... MBP-AIM2 labeled with peptide-Alexa 488 was purified by size exclusion chromatography (SEC) using Superdex 200 increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: Enzymatic degradations were monitored by analytical gel permeation chromatography using Superdex S200 10/300 and Superdex peptide 10/300 (GE Healthcare) columns mounted in series and connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2024Quote: ... The active fraction (AGH1) was further purified to homogeneity by size exclusion chromatography using a Superdex Peptide HR 10/30 (Amersham Biosciences, GE Healthcare). Elution was performed under isocratic conditio ns with 30% ACN in acidified water at a flow rate of 0.5 mL/min ...
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bioRxiv - Biophysics 2021Quote: ... dye-peptide-MHC complexes in PLB were purified using a 10,000–molecular weight cutoff spin concentrator (Vivaspin 500, GE Healthcare, Pittsburgh, PA) and 1x TBS wash [TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved SPARC peptides were separated by 13.5% or 17% SDS PAGE and analyzed by immunoblotting or silver staining (GE Healthcare Life Sciences), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using the IPGphor 3 IEF system (GE Healthcare) at 20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... 3 or 4 of a Series S CM5 chip (GE Healthcare) functionalised with an anti-His capture kit (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Plant Biology 2021Quote: The binding kinetics and affinities of MIK2ECD with SCOOP12 or SCOOP12SS/AA peptides were assessed on a Biacore T200 instrument (GE Healthcare, Pittsburgh, PA) with CM5 sensor chips ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for analyzing the binding of a synthetic GRP78-targeting peptide WDLAWMFRLPVG (GL Biochem Ltd, Shanghai, China) in Biacore X100 SPR system (GE Healthcare, NJ, USA). The system was firstly equilibrated with the running buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... dadantii OutB was cloned into the pGEX-6P-3 vector (GE Healthcare) encoding a cleavable N-terminal Glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2019Quote: ... 3 were acquired using DeltaVision Elite wide-field microscope system (Applied Precision). Images were acquired as z-stacks with a step size of 0.5 μm ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM MgCl2] using a size exclusion column (NAP5; GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 3D-SIM was carried out using an OMX Version 3 (Applied Precision) microscope equipped with a UPlan APO 100× 1.4 NA oil-immersion objective (Olympus) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using 24 cm 3–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... placed on Ficoll-Paque Plus (ratio 7:3) (GE Healthcare Life Sciences, 17144003), and centrifuged for 20 min at 1800 rpm at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Immunology 2022Quote: ... and 3 mL of 66% percoll was underlaid (GE healthcare 17–0891–01). Samples were spun for 20 min at 2000 rpm at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...