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Citations for GE Life Sciences :
51 - 100 of 142 citations for Pyruvate Kinase Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Excess reactants were removed by a Superdex peptide size-exclusion column (GE Healthcare) equilibrated in the refolding buffer ...
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bioRxiv - Biophysics 2019Quote: ... Solutions of the SGYDFEREGYS and SGYDFGREGYS peptides in PBST buffer (GE Healthcare, USA) at the indicated concentrations were used as analyte ...
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bioRxiv - Biochemistry 2020Quote: Peptides were fractionated by SCX chromatography using the AKTA Purifier system (GE Healthcare) and injected for nanoLC-MS/MS analysis ...
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bioRxiv - Biochemistry 2021Quote: ... The QconCAT peptides were purified with Ni Sepharose suspension (GE Healthcare Ltd, UK) in centrifuge filters (Corning Costar Spin-X 0.45 um pore size cellulose acetate membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescently labeled peptides were detected using a Typhoon Trio Plus imager (GE Healthcare) and band intensities were quantified using the ImageJ44 ...
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bioRxiv - Cell Biology 2023Quote: ... Free Flag peptides were removed using a PD-10 Desalting Column (GE Healthcare). The conjugate was further purified by a new PD-10 Desalting Column ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptides were desalted using a homemade C18 microcolumn (SOURCE 15RPC, GE Healthcare) and stored at -30 °C until use.
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: Eluted peptides were fractionated using a Superdex 30 Increase 3.2/300 column (GE Healthcare) at a flow rate of 10 µl/min using 30 % (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using a Superdex Peptide 10/300 GL gel-filtration column (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... Crosslinked products were enriched by size-exclusion chromatography (Superdex Peptide, GE Healthcare Life Sciences) as described previously34 and fractions eluting between 0.9 and 1.4 mL were dried ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted fluorescent peptide was removed using the Superdex 75 size exclusion column (GE Healthcare) in 50 mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Molecular Biology 2023Quote: ... The amount of [33Pγ]-tracer on the paper (signifying the kinase activity) was analyzed using a Typhoon FLA 700 IP PhosphorImager (GE Healthcare, Denmark), and related to the specific activity of the kinase reaction buffer ...
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2021Quote: ... and the excess small molecular was removed by Superdex peptide size-exclusion column (GE Healthcare) equilibrated in refolding buffer ...
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bioRxiv - Immunology 2019Quote: ... Bound biotinylated peptide was detected by addition of streptavidin-alkaline phosphatase conjugate (GE Healthcare, France) and 4-methylumbelliferyl phosphate substrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-linked peptides were enriched in a Superdex 30 Increase 3.2/300 column (GE Healthcare) equilibrated with 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography using an ÄKTAmicro chromatography system (GE Healthcare) equipped with a Superdex™ Peptide 3.2/30 column (column volume = 2.4 ml) ...
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bioRxiv - Microbiology 2021Quote: ... the peptide was purified with size-exclusion chromatography using a Superdex 75 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Na HEPES ...
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... The cargo tail peptides were isolated on gravity-flow column containing Ni-NTA agarose (GE Healthcare) followed by gel-filtration chromatography on Superdex Peptide 10/300 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was then separated from the Cy3-peptide using a second heparin column (GE Healthcare) and polished using a MonoQ 5/50 GL (GE Healthcare) ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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bioRxiv - Immunology 2021Quote: ... or other peptide–HLA-A2 ligand was immobilized on a streptavidin-coated BIAcore SA chip (GE Healthcare) at around 1000 resonance units (RU) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
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bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
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bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Biophysics 2021Quote: ... They were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – #SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and were cultured in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... This solution was purified by size exclusion chromatography using a superdex peptide column (10/300, GE Healthcare, Germany) in 20 mM phosphate pH 7.4 buffer containing 4M GdnCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 2F2 Fab-peptide complexes were purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare), concentrated and diluted to 10 mg/mL ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... the sensor peptide was purified by size exclusion chromatography using the Superdex 75 10/300 GL column (GE Healthcare) and then stored at −80 °C before use.
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bioRxiv - Cancer Biology 2022Quote: ... peptides from whole cell samples were focused on immobilized pH gradient (IPG) gel strips (GE Healthcare, Waukesha, WI, USA) on a linear pH range of 3-10 ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
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bioRxiv - Microbiology 2022Quote: ... The solution containing the peptide was applied to a HiPrep SP FF 16/10 column (GE Healthcare Life Sciences) equilibrated with 20 mM sodium phosphate buffer at pH 3.9 ...