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Citations for GE Life Sciences :
351 - 400 of 6288 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The protein-protein complex was then passed through a size-exclusion column (Superdex75, 26/60, GE Healthcare) to remove unbound proteins ...
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bioRxiv - Microbiology 2021Quote: ... Secreted proteins were purified from the culture supernatants using a protein A+G affinity column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Molecular Biology 2021Quote: ... and the assay buffer was HBS-EP+ buffer (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... ITC binding assay was performed via an ITC200 (GE Healthcare) equipment at 25 °C ...
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bioRxiv - Microbiology 2023Quote: Column-based binding assay used heparin-agarose beads (GE Healthcare) as described (48 ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was neutralized by addition of 1:10 vol:vol 1M Tris pH 8 and applied to a 5 mL protein A Hitrap FF column (GE Healthcare Life Sciences) equilibrated in buffer A (100 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... previously calibrated with protein standards (GE Healthcare), including thyroglobulin (669 kDa) ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).