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Citations for GE Life Sciences :
301 - 350 of 433 citations for PD L1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
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bioRxiv - Bioengineering 2020Quote: ... Unlabelled radiometal complexes were removed by separating the vesicles with PD-10 columns (GE Healthcare Life Sciences™). Both vesicles were concentrated using Pierce™ Concentrators (30 kDa ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... Residual NaCO3 from the reverse phase were removed using a PD-10 desalting column (GE Healthcare Life Sciences). Before aggregation ...
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bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
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bioRxiv - Biochemistry 2022Quote: ... followed by further separation from small molecules by passage through a pre-packed PD-10 column (GE Healthcare). Protein concentrations were determined by absorbance at 280 nm using a calculated molar extinction coefficient (ε280nm ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: Ni-NTA eluates were thawed on ice and desalted via the PD-10 desalting column system (GE Healthcare) following the manufacturer’s gravity flow protocol ...
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bioRxiv - Pathology 2020Quote: ... free TPEN and free Zn were further removed by gel filtration on PD MidiTrap G-10 (GE Healthcare) equilibrated with 40 mM of the metal-free HEPES-KOH ...
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bioRxiv - Microbiology 2019Quote: ... The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare) equilibrated with 20 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... 500 mM NaCl using a gravity-flow chromatography with a PD-10 desalting column (GE Healthcare Life Sciences). Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... The buffer was exchanged with PBS by size exclusion chromatography using a PD-10 Desalting Column (GE Healthcare). The purity of EqPD-1-Ig and EqPD-L1-Ig was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions corresponding to pure RIP2K were then pooled and applied onto a PD-10 desalting column (GE Healthcare) equilibrated with buffer C (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: RIP2K1-317 buffer exchange in buffer F was done by using a PD-10 desalting column (GE healthcare). 5mM MgCL2 and 2 mM ATP were added to the kinase sample ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.[58] Desalting was carried out using a PD-10 column (GE Healthcare Life Sciences, UK). For PCMjMAT the protein was further purified by heating at 80 °C for 20 min followed by 30 min centrifugation at 18213 × g (Eppendorf centrifuge 5427 R) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was purified using size-exclusion chromatography (PD MidiTrap G-25, GE Healthcare Bio-Sciences, gravity protocol) and stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Buffer exchange was performed using 5E10 VPs and PD-10 MiniTrap G-25 columns (GE Healthcare, Solingen, Germany) following the companies instructions with the “gravity” protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were desalted into storage buffer (50mM Tris [pH 8.0], 0.2M NaCl, 0.5mM EDTA) using PD MiniTrap Sephadex G-25 columns (GE Healthcare). Otherwise ...
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bioRxiv - Biochemistry 2023Quote: ... Imidazole and NaCl were removed using a PD-10 desalting column according to the manufacturer’s instructions (GE Healthcare) at 4 °C and the concentration was determined by spectrometry (NanoDrop Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... followed by DTT removal using a PD-10 desalting column with Sephadex G-25 resin (from GE healthcare). The reduced protein was mixed with a ten fold excess of the nitroxide spin label MTSL and incubated at room temperature protected from light for one hour ...
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bioRxiv - Biophysics 2020Quote: ... Concentrated fraction was mixed with another fraction and was loaded into PD-10 desalting column (GE Healthcare Lifescience, 17085101) for buffer exchange with incubation buffer containing 50 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... the preparation was diluted in PBS and applied to a Sephadex G-25 column in PD-10 (GE Healthcare). Particle concentration and size distribution of the liposomes generated were evaluated using the NanoSight NS300 instrument (Malvern ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded on a equilibrated G-25 column (Column PD Minitrap G-25, GE Healthcare, Cat.# 28-9180-07). Once the solution fully entered the column ...
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bioRxiv - Biochemistry 2020Quote: Lipoproteins secreted into the basolateral media were separated from free tracers using a PD-10 desalting column (GE Healthcare) as described previously [23] ...
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bioRxiv - Genomics 2021Quote: ... DNA from the isolated pooled LL and HL fractions was desalted on PD MiniTrap G-25 columns (GE Healthcare) equilibrated in 10m Tris-Cl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... LUVs were subjected to size exclusion chromatography on a sephadex G25 PD-10 column (GE Life Sciences, Chicago, Il). The concentration of the encapsulated Indo-1 was estimated using fluorescence and known amounts of free Indo-1 ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Biophysics 2019Quote: ... Free NBD dye was removed by gel filtration using a PD-10 column (GE Healthcare Life Sciences, Marlborough, MA), while labeled and unlabeled peptide were separated by reverse-phase HPLC (Agilent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
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bioRxiv - Molecular Biology 2020Quote: ... sample was desalted into Fab-storage buffer (20 mM HEPES pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fab30 was flash frozen stored in buffer supplemented with 10% glycerol.
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bioRxiv - Biochemistry 2020Quote: ... The conjugated Fab were then eluted from a PBS pre-equilibrated PD-mini G-25 desalting column (GE Healthcare) that removed unconjugated dye ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The ACP-TNB− was isolated and desalted using a Sephadex G-25 PD-10 desalting column (GE Healthcare, #17085101) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted samples were desalted into buffer (20 mM HEPES-Na+, pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fabs were flash-frozen and stored at −80 °C supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Free dye was removed with buffer exchange to carbonate-bicarbonate buffer (0.2 M NaHCO3 – Na2CO3, pH 8.5) using size-exclusion chromatography (PD-10 Desalting columns, GE Healthcare). Next ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... The unconjugated DFO was removed from the antibody-DFO conjugates using a size exclusion column (PD-10; GE Healthcare) and the antibody solutions were concentrated using amicon filters with a 50 kDa cutoff filter ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... elutes of KLP10A were subjected to buffer exchange to 1xMRB80 with 75 mM KCl and 0.1 mM ATP using a PD-25 column (GE Healthcare). AtMIDD1 was purified following (Oda and Fukuda ...
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to 50 mM HEPES pH 7.0 containing 150 mM NaCl and 10 mM CaCl2 (Reaction Buffer) using a PD-10 desalting column (GE Healthcare). Aliquots containing 240 µg total protein were incubated at 37 C in the presence of 750 nM MMP2 or 9 or the Reaction Buffer alone for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...