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Citations for GE Life Sciences :
101 - 150 of 2251 citations for PD 1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... PD-10 desalting columns were from GE Healthcare (Port Washington, NY). All other solvents and reagents were from either VWR or Sigma.
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... at a 1:3 protein:dye molar ratio for 1 h at room temperature and then desalted on a PD-10 desalting column (GE Healthcare), where the free dye and the conjugated Alexa488-αSOs were separated ...
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bioRxiv - Microbiology 2019Quote: ... Excess oxidant was removed by a PD-10 desalting column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... the buffer was exchanged via a PD-10 desalting column (GE Healthcare) into 10 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1mM DTT) by gel filtration with PD MidiTrap G10 columns (GE healthcare) according to instructions of manufacturers.
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bioRxiv - Cell Biology 2022Quote: ... The eluate was loaded onto a PD-10 desalting column (GE Healthcare) and eluted with PBS to remove salt ...
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bioRxiv - Neuroscience 2019Quote: ... The purified proteins were finally desalted on PD-10 columns (GE Healthcare) in 50 mM HEPES/NaOH buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein was desalted using a PD-25 column (GE Healthcare), eluting with 50 mM potassium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... free calcein was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the dialysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... free dye was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer (5 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Vipp1L193C or Vipp1L86C/L193C using PD MiniTrap G-25 columns (GE Healthcare) equilibrated in 25 mM Tris-HCl pH 8.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Then protein samples were desalted through a PD-10 column (GE Healthcare). In vitro spectroscopy was performed in 50 mM ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 mM DDM using a PD-10 column (GE Healthcare Bio-Sciences). The sample was concentrated to 1.0 mg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.005% GDN by using PD-10 columns (GE Healthcare Life Sciences), and air bubbles were removed by centrifugation for 5 min at 10,000 g prior to ITC ...
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bioRxiv - Biochemistry 2022Quote: ... After exchange into wash buffer using PD-10 desalting columns (GE Healthcare) His tags were cleaved with thrombin (1 U/mg EmrE3 ...
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bioRxiv - Biophysics 2022Quote: ... followed by desalting with a PD MiniTrap G-10 column (GE Healthcare) to remove the excess unreacted label ...
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bioRxiv - Biophysics 2022Quote: ... followed by desalting with a PD MiniTrap G-10 column (GE Healthcare) to remove the excess unreacted biotin ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatants were used directly or filtered through PD-10 columns (GE Healthcare) and incubated with streptavidin (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was desalted using PD spin trap columns (GE Healthcare, USA), stored at −80 °C until use and protein concentration was determined by Bradford protein assay using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was passed over a PD-10 column (GE Healthcare, Chicago, Illinois) equilibrated with PBS ...
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bioRxiv - Microbiology 2020Quote: Imidazole was removed using a PD-10 Desalting Column (GE Healthcare, UK) with an imidazole-free equilibration buffer (50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2021Quote: ... and the buffer was exchanged using PD-10 column (GE Healthcare, USA) to Buffer-E (20 mM HEPES (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... 150 mM NaCl and 0.012% LMNG with PD-10 columns (GE healthcare) for the removal of imidazole ...
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bioRxiv - Synthetic Biology 2021Quote: ... imidazole desalting was performed using PD-10 desalting columns (GE Healthcare #17085101) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing rCovR were pooled and desalted (PD-10 columns, GE Healthcare) with 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... Excess dye was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Excess calcein was removed with a desalting column (PD-10, GE Healthcare) that had been equilibrated with the dialysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... Purified protein was desalted with a PD-10 desalting column (GE Healthcare) in storage buffer (20 mM Tris-Cl ...
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bioRxiv - Plant Biology 2023Quote: ... the TCEP was removed using a PD-10 desalting column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Resin was poured into 10 mL empty PD-10 columns (GE Healthcare) and all washes and elution were performed by gravity flow with buffers as used for WT purification ...
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bioRxiv - Biochemistry 2023Quote: ... EDTA and DTT were removed via a PD-10 column (GE Healthcare) equilibrated with 50 mM HEPES (pH 7.5) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified protein was buffer exchanged with PD-10 columns (GE Healthcare) into 100 mM potassium phosphate buffer pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... The product was purified using PD-10 columns (GE Healthcare, Chicago, IL) in deionized water containing 3 mM BME ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4) using a PD-10 desalting column (GE Healthcare Life Sciences).
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bioRxiv - Genetics 2022Quote: ... and desalted using a PD-10 column (GE Healthcare, Pittsburgh, PA, USA) to remove imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was buffer exchanged a PD-10 desalting column (GE Healthcare) that had been equilibrated with RIPA buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... all proteins were desalted using PD MiniTrap G-25 columns (GE Healthcare) and protein quality was checked by CBB staining and immunoblotting ...
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bioRxiv - Immunology 2023Quote: ... Excess unconjugated dye was removed using PD-10 desalting columns (GE Healthcare). pHrodo Red-labeled antibodies were exchanged into HEPES buffer and concentrated in an Amicon Ultra centrifugal filter unit with a nominal molecular weight cutoff of 30 kDa ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each Hb solution was then desalted using a PD-10 column (GE Healthcare) equilibrated with 0.01 M HEPES/0.5mM EDTA (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5% glycerol) using PD SpinTrap G-25 columns (GE Healthcare #28-9180-04) pre-equilibrated with conjugation buffer ...
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bioRxiv - Biochemistry 2022Quote: ... pH 4.0 by gel filtration chromatography using a PD-10 column (GE Healthcare), and concentrated to a final protein concentration of 1.0 – 1.5 mM in 250 μL using a Vivaspin microfiltration device and placed in a 5 mm Shigemi reduced volume NMR tube for measurements ...
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bioRxiv - Biochemistry 2020Quote: ... Desalting/buffer exchange was performed using a PD-10 desalting column (GE Healthcare), where HspA1A fractions were transferred into anion-exchange wash buffer (50mM Tris ...
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bioRxiv - Physiology 2019Quote: ... The sample was then passed through a 5 ml PD-10 (GE Healthcare) desalting column equilibrated with 10 mM Hepes ...