Labshake search
Citations for GE Life Sciences :
301 - 350 of 2685 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
-
bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Excess dye was removed using a PD-10 desalting column (GE Healthcare, Chicago, IL, USA). Titrations were performed in PBS using a constant 1 μM concentration of fluorescein-coupled peptide with increasing concentration of R7R8 fragment (either wild type or vvv mutant) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The H4 protein was separated and desalted using a PD-10 desalting column (GE Healthcare), preequilibrated in water supplemented with 1mM acetic acid and lyophilized ...
-
bioRxiv - Bioengineering 2019Quote: ... The generated ADC was purified with PD SpinTrap™ G25 column (GE Healthcare, Chicago, IL) or high-performance liquid chromatography (Waters ...
-
bioRxiv - Biophysics 2019Quote: ... The other was buffer exchanged into SEC buffer using a PD-10 column (GE Healthcare The protein was then treated with 0.4 units of bovine kidney α-L-fucosidase (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... pH 6.8) supplemented with 10mM DTT and 50mM NaCl using PD-10 columns (GE healthcare). Protein concentration was measured using standard Bradford method ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
-
bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...
-
bioRxiv - Biochemistry 2020Quote: ... columns from Thermo Scientific [Cat# 60106-303] and PD Minitrap Sephadex G-10 size exclusion cartridges from GE Healthcare [Cat# 28-9180-10] ...
-
bioRxiv - Biochemistry 2020Quote: ... Unreacted dye was removed by passing through the PD MidiTrap G-25 column (GE Healthcare) three times ...
-
bioRxiv - Biophysics 2020Quote: ... imidazole was removed using a PD-10 desalting column (GE Healthcare, Pittsburgh, PA, USA; # 17085101).
-
bioRxiv - Biophysics 2020Quote: ... and applied to a PD-10 gel filtration column (GE Healthcare Ltd, Little Chalfont, UK), which had been previously pre-equilibrated with the LSNMR buffer ...
-
bioRxiv - Biophysics 2020Quote: ... and the buffer was exchanged into final buffer by PD-10 desalting column (GE Healthcare). The final protein concentration was determined by UV-Vis absorption at 274 nm using an extinction coefficient of 2.8 cm-1mM-1 for tau187 and 5.8 cm-1mM-1 for 2N4R ...
-
bioRxiv - Biochemistry 2022Quote: ... ±0.005 mg/mL tetraoleoyl cardiolipin) using a PD-10 column (17-0851-01; GE Healthcare) and protein quantified (BCA assay ...
-
bioRxiv - Biophysics 2022Quote: ... the protein was eluted by gravity flow using a PD MiniTrapTM G-25 (GE Healthcare) Sephadex column into 200 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... the supernatant was further de-proteinized employing Sephadex G-25 Column (Pd-10, GE Healthcare) loaded with the same extraction solution ...
-
bioRxiv - Synthetic Biology 2022Quote: ... followed by desalting with a PD-Midi trap G-25 column (GE Healthcare Life Sciences). The constructs containing enzymes were desalted into 50 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... pH 7.5 using a PD-10 desalting column with Sephadex G-25 resin (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... PD-10 columns Sephadex G-25 (20×80 mm) were from GE Healthcare (cat# 17085101).
-
bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
-
bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Sephadex G-25 in PD-10 desalting columns were purchased from GE Healthcare (Buckinghamshire, UK). Fluorescein isothiocyanate (FITC)-conjugated plain ...
-
bioRxiv - Biochemistry 2023Quote: ... and DTT was removed by gel filtration on PD Minitrap G-25 columns (GE Healthcare) after column equilibration with strictly degassed buffer.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and extravesicular ANTS was removed using a PD-10 desalting column (GE Healthcare, cat # 17085101). The rate of ANTS fluorescence quenching by the gA channel-permeant Tl+ was recorded with an Applied Photophysics SX.20 (Leatherhead ...
-
bioRxiv - Biophysics 2023Quote: ... The labeled BRICHOS was separated from unreacted label by a PD-10 column (GE Healthcare) equilibrated with PBS ...
-
Balanced Interplay Between Hsp110, Hsp70 and Class B J-Domain Protein Improves Aggregate DisassemblybioRxiv - Biochemistry 2023Quote: ... An excess of the label was removed with a desalting column (PD-10, GE Healthcare). The BLI experiment was performed as with unlabelled proteins and the fluorescence of the protein dissociated into the buffer A with 5 mM ATP 1 mM DTT was measured using Beckman Coulter DTX 880.
-
bioRxiv - Biochemistry 2023Quote: ... The eluted product was dialyzed with PD-10 columns according to manufacturer’s protocol (GE Healthcare) to Trp Aptamer buffer (TAB ...
-
bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
-
bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
-
bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
-
bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
-
bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
-
bioRxiv - Immunology 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) using a PD-10 desalting column (GE Healthcare, USA). Light chain of human enterokinase (10 μg/ml ...
-
bioRxiv - Plant Biology 2019Quote: ... and the imidazole was removed using Sephadex G-25 PD-10 Desalting Columns (GE Healthcare, Uppsala). Protein purity was analyzed by SDS-PAGE gel and protein masses were confirmed by intact mass spectrometry.
-
bioRxiv - Microbiology 2019Quote: ... Buffer exchange of the purified protein was performed by using PD-10 desalting column (GE Healthcare).
-
bioRxiv - Cancer Biology 2020Quote: ... unbound DFO-Bz-NCS was removed with a PD-10 desalting column (GE Healthcare, Piscataway, NJ) using NH4OAc buffer (0.15 M ...
-
bioRxiv - Biochemistry 2020Quote: ... overnight and protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was applied to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare) equilibrated in deoxygenated chelex-treated buffer (10 mM HEPES pH 7.0 ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The eluent containing 300 mM imidazole buffer was desalted using PD-10 desalting column (GE Healthcare) with buffer (20 mM Tris-HCl ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The eluent containing 300 mM imidazole buffer was desalted using PD-10 desalting column (GE Healthcare) with buffer (20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2019Quote: ... according to the instructions of the manufacturers and purified using PD-10 desalting columns (GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... the loaded GTPase was purified using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in the final GTPase buffer (20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2020Quote: ... 55Fe-transferrin was separated from unbound 55Fe by running on PD-10 desalting column (GE Healthcare). For uptake experiment ...