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Citations for GE Life Sciences :
351 - 400 of 922 citations for Nipah virus Nucleoprotein N His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were dried on Amersham Hybond-N+ membrane (GE Healthcare) and analyzed using a Typhoon FLA 7000 biomolecular imager ...
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bioRxiv - Genomics 2019Quote: ... and electroblotted/UV crosslinked to Hybond N+ membrane (GE Healthcare RPN303B). ULTRAhyb-oligo hybridization Buffer (Thermo Fisher Scientific AM8663 ...
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bioRxiv - Genomics 2019Quote: ... RNAs were electroblotted onto nylon membranes (Amersham Hybond-N, GE Healthcare) and UV-fixed with a crosslinker (UVC 500 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then loaded onto a Hybond-N+ membrane (GE Healthcare), allowed to air dry and crosslinked twice at 200000 μJ/cm2 UV ...
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bioRxiv - Plant Biology 2019Quote: ... digested DNA was transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labelled specific DNA probe for the HYG gene and signals were detected using CDP-Star according to the manufacturer’s instructions (Roche Diagnostics)
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bioRxiv - Molecular Biology 2019Quote: ... and then transferred to Amersham Hybond-N+ membrane (GE life sciences). After UV crosslinking and baking ...
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bioRxiv - Molecular Biology 2019Quote: ... which was transferred onto a Amersham Hybond−-N+ membrane (GE Healthcare), crosslinked on a UV Stratalinker 2400 ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel was transferred to a Hybond N+ membrane (GE Healthcare) over-night (O/N ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was transferred onto a Hybond-N+ membrane (GE healthcare) and UV-cross-linked (Stratagene Stratalinker 1800 ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto Amersham Hybond™-N+ membrane (GE Healthcare Cat#RPN203B) and hybridized to radiolabelled oligonucleotide probes (Table S3 ...
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bioRxiv - Immunology 2021Quote: ... was applied to the Amersham Hybond-N+ membrane (GE Healthcare, USA) containing RNA samples ...
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bioRxiv - Biochemistry 2020Quote: The N-terminus of Rad6 was labeled with Cy5 (GE Healthcare). Briefly ...
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bioRxiv - Genetics 2020Quote: ... and DNA was transferred to a Hybond-N membrane (GE Healthcare) by blotting ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then transferred to Amersham Hybond-N+ blot (GE Healthcare) by capillary transfer in 10 x SSC and crosslinked to the blot by UV (254 nm ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transferred to Hybond-N+ membranes (GE Healthcare, Cat #RPN303B) by the capillary transfer method using blotting paper ...
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bioRxiv - Plant Biology 2023Quote: ... and transferred onto an Amersham Hybond N+ nylon membrane (GE Healthcare). The 840 bp HPT probe was synthesized by PCR DIG Probe Synthesis Kit (Roche ...
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bioRxiv - Immunology 2019Quote: ... His10-tag free CD80 ectodomain was purified with a StrepTrap HP column (GE Healthcare, catalog 28907547) in 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the protein without His6-tag was injected into a Superdex 75 10/300 GL (GE Healthcare) size exclusion column with the buffer B (50 mM NaH2PO4/Na2HPO4 pH 6.5 ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl of equilibrated in lysis buffer His Mag Sepharose beads Ni (GE28-9799-17, GE Healthcare) was added to each sample and kept on a roller overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the 6x His-MBP fusion was further purified on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration chromatography with Superdex-75 SEC column (Hi-Load 16/600, Prep Grade, GE Healthcare, USA) and MALDI analysis were used to determine the molecular mass of PrASNase [23].
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...