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Citations for GE Life Sciences :
201 - 250 of 4135 citations for Native Red Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The amount of Aβ42 bound to the cells was measured as mean fluorescent intensity using In Cell 6000 microscopy (GE healthcare, USA). The specific binding of Aβ42 was calculated by subtracting the fluorescence of cells in the absence of Aβ42 from that of fluorescence in the presence of varying concentrations of Aβ42 ...
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bioRxiv - Microbiology 2020Quote: ... Super-resolved fluorescent images were reconstructed with the corresponding recorded optical transfer function (OTF) in the SoftWoRx 7.0.0 software (GE Healthcare, Amersham, UK) at a Wiener filter setting of 0.006.
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bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
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bioRxiv - Physiology 2019Quote: ... Analysis of fluorescent signals was performed using the Typhoon Trio laser scanner (λem=526nm, λex= 488 nm) and the ImageQuant software (GE Healthcare). TLC plates were stained with 470 mM CuSO4 in 8.5% o-phosphoric acid and subsequently incubated for 10 min at 180°C.
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bioRxiv - Biochemistry 2021Quote: ... and the digestion products were then loaded in 7 M urea containing 20% (wt/vol) polyacrylamide gels (acrylamide/bisacrylamide ratio 19:1) buffered with TBE and visualized using a Fluorescent Image Analyzer Typhoon (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed 3x for 8min with TBST and the fluorescent signal from the membranes was recorded with an Amersham Typhoon (GE Healthcare). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Cell Biology 2022Quote: Fibronectin was purified from outdated human plasma (American Red Cross, Rochester, NY) using gelatin-Sepharose (GE Life Sciences, now Cytiva) affinity chromatography (102) ...
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bioRxiv - Immunology 2019Quote: ... or neutrophils were isolated by dextran (250,000) sedimentation of red blood cells (RBC) followed by Ficoll-Paque PLUS H-1077 (GE Healthcare) gradient separation as described before with modification (Denny et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Minced mammary tissues were then digested in phenol-red free Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque) containing 2mM L-glutamine (GE healthcare), 1mg/ml collagenase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging of MCF-10A cells was completed using the Applied Precision DeltaVision OMX fluorescent microscope (Applied Precision, GE Healthcare, Issaquah, WA, USA). Images shown are representative images at 60X magnification ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging of MCF-10A cells was completed using the Applied Precision DeltaVision OMX fluorescent microscope (Applied Precision, GE Healthcare, Issaquah, WA, USA). Images shown are representative images at 60X magnification ...
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bioRxiv - Cell Biology 2021Quote: Image acquisition of Hoechst 33342 fluorescent nuclei (excitation: 361nm to 497nm; emission 460nm to 490nm) was performed using the INCell analyzer 6500HS automated system (GE Healthcare, USA) at a 10X magnification (Nikon 10X/0.451 ...
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bioRxiv - Cell Biology 2020Quote: ... Total internal reflection fluorescent (TIRF) images were acquired using the DeltaVision Elite microscopy system equipped with a multi-line TIRF module (Applied Precision, Inc.), and time-lapse movies were taken at 30-seconds intervals ...
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bioRxiv - Cell Biology 2022Quote: For whole mount staining of embryonic hearts Ad2 was directly labelled via an NHS ester by the fluorescent dye Cy2 according to the instructions of the manufacturer (GE Healthcare, #32000). Ad2-Cy2 was applied at a concentration of 6 μg/ml in PBS/0.1%Tx100/5% goat serum for 2 hr at room temperature to paraformaldehyde fixed E10.5 hearts followed by several washing steps ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... previously calibrated with protein standards (GE Healthcare), including thyroglobulin (669 kDa) ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hrs at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies (antibodies listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... or agarose Protein G beads (GE Healthcare) only ...
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bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
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bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the pads were sealed with a coverslip and fluorescent microscopy was performed with a DeltaVision Spectris Deconvolution Microscopes (Applied Precision, Issaquah, WA, USA). Cells were imaged for at least 8 stacks in the Z-axis from the middle focal plane with 0.15 μm increments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Immunology 2024Quote: 1μg of purified fosmid/BAC DNA purified using standard alkaline lysis protocol was labelled with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science) in a 50μl nick-translation reaction for 3h at 15°C.