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Citations for GE Life Sciences :
101 - 132 of 132 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Genetics 2023Quote: Knockdown of mouse Klf4 transcripts was performed with an ON-TARGETplus SMARTpool siRNA against mouse Klf4 (L-040001-01-005, 16600) (Dharmacon, GE Healthcare). This pool of four targeting RNAs targets the following sequences ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
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bioRxiv - Neuroscience 2020Quote: ... concentrated and further purified by size-exclusion chromatography on a Superdex 200 26/60 column L (GE Healthcare, Cat.# 28-9893-36). Purified scFv-Clasp were flash frozen in aliquots (in 10 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Biochemistry 2019Quote: ... the concentrated linked-L-protein from the Q-Sepharose eluate was subsequently fractionated over a Sephadex S200 26/600 PG (GE Life Sciences) column using STD buffer (100 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run on the gel at 300V for 135 minutes using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue).The samples were transferred to a Hybond-XL membrane (GE healthcare, RPN203S) at 50V for 1 hour at 4°C and then cross-linked using 0.12J (UV-lamp T8C ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of each field sample was subject to whole genome amplification using the illustra Genomiphi V2 DNA amplification kit (GE Healthcare Life Sciences, Australia) for 2 hours at 30°C followed by heat inactivation at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl were applied to an Äkta pure chromatography system equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) and run at 6 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Antibiotics were dissolved to a final concentration of 1280 mg/L and filtered using a cellulose acetate membrane (0.2 µm pore size, GE Healthcare Life Science, Freiburg, Germany) to ensure sterility ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Neuroscience 2021Quote: ... IRDye® 680LT goat anti-rabbit IgG (H + L) (LiCor, cat. #926-6802, 1:10,000) and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (GE Healthcare, cat. #AS09603, 1:10,000).
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bioRxiv - Biophysics 2021Quote: ... They were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – #SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and were cultured in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HESCs were grown in a six-well culture plate to 60%–70% confluence and transfected with 60 pmol of non-targeting siRNA (D-001810-10-05) or siRNAs targeting ACE2 (L-005755-00-0005) (GE Healthcare Dharmacon Inc., Lafayette, CO) in Lipofectamine 2000 reagent (Invitrogen Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... 150 μl of DEPC-treated water was then added and the reaction passed through a home-poured G-25 sephadex (GE Healthcare cat. # 17-0033-01) spin column ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were then eluted with 100 mM L-glutathione reduced and subjected to a second purification by addition of Nickel-Sepharose beads 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA). The protein was eluted with HKI500 buffer (20 mM Hepes ...
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bioRxiv - Biochemistry 2022Quote: ... the remaining 200 µL were snap-frozen in liquid nitrogen and stored at –80 °C) were isolated through MicroSpin S-400 columns (GE Healthcare, Cat. No. 27-5140-01), and 20 µL of 10% SDS were added ...