Labshake search
Citations for GE Life Sciences :
251 - 300 of 1520 citations for Mouse S formylglutathione hydrolase Esd ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... and then transferred onto nitrocellulose membrane Watman® Optitran® BA-S 85 (GE Healthcare, Castle Hill, NSW, Australia). The membrane was blocked (1 h at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: Differential scanning calorimetry (DSC) was carried out on the S proteins using a MicroCal VP-DSC instrument (GE Healthcare, MicroCal Products Group ...
-
bioRxiv - Biophysics 2022Quote: ... dialyzed against 20 mM MOPS-NaOH pH 6.7 and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated with the same buffer ...
-
bioRxiv - Biophysics 2019Quote: ... and ClpAD1 was purified by ion-exchange chromatography using S-Sepharose and MonoQ HR 5/5 columns (GE Healthcare). Purified ClpAD1 was dialyzed into storage buffer (50 mM HEPES ...
-
bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
-
bioRxiv - Biophysics 2019Quote: ... The K48-diUb protein was purified from the reaction mixture using Superdex 75 and Source-S columns (GE Healthcare) in tandem ...
-
bioRxiv - Biochemistry 2021Quote: ... The flow-through was further purified by cation-exchange chromatography on a 5 mL HiTrap S column (GE Healthcare) with a salt gradient of 0 to 1 M NaCl in 1×PBS supplemented with 1 mM DTT ...
-
bioRxiv - Microbiology 2020Quote: ... ∼500 µL of concentrated pooled fractions was loaded onto HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare) then 1.5 column volume of mobile phase (DPBS ...
-
bioRxiv - Neuroscience 2021Quote: ... hTau and mTau were immobilised onto an amine-coupling activated (NHS/EDC) CM5 Series S Sensor Chip (GE Healthcare) with a contact time of 180 s at 5 µL/min flow rate and at 25°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
-
bioRxiv - Biochemistry 2023Quote: ... 30 mM NaCl (buffer A) the supernatant was loaded onto a Mono S cation exchange chromatography column (GE Healthcare) equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2019Quote: ... or anti-phospho hTERT mouse mAb (TpMab-1) and anti-mouse IgG HRP (GE Healthcare) were used for IB to detect phosphorylated hTERT proteins.
-
bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
-
bioRxiv - Cell Biology 2019Quote: ... Metaphase cells were imaged for 5 min every 7.5 s using an Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma) ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was then run over a size exclusion column (GE Healthcare HiPrep™ 26/60 Sephacryl S-300 HR) in sizing buffer (50mM Tris-HCl pH=8.0 ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein-containing fractions were concentrated and further purified on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... the protein was purified by size exclusion chromatography over a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... The supernatant was 0.45-μm filtered and injected into a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare). SEC elution profiles were monitored by measuring absorbance at λ=280 nm ...
-
bioRxiv - Biochemistry 2021Quote: ... Fp231 was further purified by gel filtration (HiPrep™ 16/60 Sephacryl™ S-200 HR; GE Healthcare, Uppsala, Sweden) in 50 mM sodium citrate-phosphate pH 6.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... the protein was further purified over a gel filtration column (HiPrep 16/60 Sephacryl S-100 High Resolution, GE Healthcare) equilibrated with AUC buffer (50 mM NaH2PO4 pH 7.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... The flow-through from this step was concentrated in an Amicon Ultra Centrifugal Filters (10,000 MWCO, Milipore) and applied to a HiPrep 16/60 Sephacryl S-200 gel filtration column (GE Healthcare) in buffer containing 50 mM HEPES (pH 7.5) ...
-
bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
-
bioRxiv - Microbiology 2020Quote: ... Digestion was stopped with SuperaseIN and monosomes purified by size exclusion chromatography on MicroSpin S-400 HR columns (GE Healthcare) as described (Shamimuzzaman ...
-
bioRxiv - Immunology 2022Quote: ... The sensorgrams of the three rounds were aligned from 120 to 240 s in Biacore 8K Evaluation software (GE Healthcare). The blocking efficacy was determined by a comparison of the response units with and without prior antibody injection.
-
bioRxiv - Biochemistry 2020Quote: ... concentrated and was followed by size exclusion chromatography (SEC) with a Superdex column (S-200, 16/60, GE life sciences) pre-equilibrated with buffer C (Tris-Cl 50 mM ...
-
bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
-
bioRxiv - Biochemistry 2020Quote: ... The measurements were done at flowrate of 0.5 ml/min over an S-75 Superdex 10/300 column (GE Healthcare) in 20 mM Tris pH 7.4 ...
-
bioRxiv - Microbiology 2021Quote: ... the protein precipitate was resuspended in 50 mM MOPS buffer (pH 7.0) and then applied to a Sephacryl S-100 column (GE Healthcare) equilibrated with the same buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... Free dye was removed by size exclusion chromatography using a HiPrep 16/60 Sephacryl S 100 HR column (GE Healthcare) equilibrated with PBS buffer ...
-
bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
-
bioRxiv - Molecular Biology 2021Quote: ... All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque Inc.) supplemented with 10% v/v fetal bovine serum (FBS; Hyclone, GE Healthcare) and penicillin/streptomycin (Nacalai Tesque Inc. ...
-
bioRxiv - Biophysics 2022Quote: ... The refolding mixture was dialyzed at 4 °C against 20 mM MOPS-NaOH pH 6.7 to remove residual GdmCl and applied to a Resource S column (GE Healthcare) to separate FimCHisI from free FimCHis ...
-
bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
-
bioRxiv - Microbiology 2023Quote: ... The eluted fractions containing Cas9-NLS-His were diluted 4-fold with 50 mM Tris-HCl (pH 7.5) and subjected to cation exchange chromatography (Hitrap S HP, GE healthcare) pre-equilibrated with 50 mM Tris-HCl (pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
-
bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
-
bioRxiv - Biochemistry 2023Quote: ... The respective reactions were stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
-
bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
-
bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Each protein was then purified on a HiPrep™ 16/60 Sephacryl S-500 HR gel filtration column (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5) ...
-
bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
-
bioRxiv - Genomics 2023Quote: ... 50 ml of sea water was filtered through a 1.2 μm pore-size acrodisc (FP 30/1.2 CA-S cat N° 10462260 Whatman GE Healthcare Sciences) and used to inoculate culture flasks with 10 ml of filtrate each ...
-
bioRxiv - Biochemistry 2024Quote: ... proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Optitran BA-S 85, pore size 0.45 µm, GE Healthcare) using a semi-dry blotting chamber at a constant current of 140 mA/gel for 60 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... The cleaved SipAC was then passed through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) using actin polymerization buffer [F-buffer ...
-
bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
-
bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...
-
bioRxiv - Immunology 2019Quote: ... donkey anti-mouse-HRP (GE Healthcare), donkey anti-rabbit-HRP (GE Healthcare) ...