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Citations for GE Life Sciences :
201 - 250 of 5201 citations for Mouse MBL2 Binding Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... SPR binding experiments were performed with a BIAcore 3000 instrument and analyzed with BIAevaluation software v4.1 (GE Healthcare). CM5 chips (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Microbiology 2020Quote: ... The response data were fit to a 1:1 binding model using Biacore™ Insight evaluation software (GE Healthcare). For Fab competition experiments (Figure S8) ...
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bioRxiv - Microbiology 2019Quote: Direct binding of His6-SidI to SusF was assessed by SPR using a Biacore T-200 instrument (GE Healthcare) at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). The equilibrium dissociation constants (binding affinity ...
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bioRxiv - Microbiology 2021Quote: ... Data were double reference-subtracted and fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Sensorgrams were fitted using 1:1 binding model and kinetic constants determined using Biacore T200 Evaluation software (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: The binding analysis of the substrate analog AMP-PNP was carried out in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva) ...
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Synthetic Biology 2019Quote: ... and regression curves were fitted based on a one-binding site model using the Microcal Origin software (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... gels were firmly attached to one of the two glass gel plates via binding silane (GE Healthcare, Uppsala, Sweden) whereas the opposite plate was coated with a releasing agent (Blueslick ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of GDAP1Δ319-358 and GDAP1Δ295-358 towards HA was measured using a MicroCal iTC200 calorimeter (GE Healthcare) in SEC buffer with 2% (v/v ...
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bioRxiv - Biophysics 2021Quote: SecA:lipid binding experiments were performed using L1 sensor chip on two-channel Biacore X100 instrument (GE Healthcare Life Sciences). DOPC-only liposomes lacking SecA interaction were employed in the reference channel ...
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bioRxiv - Cell Biology 2021Quote: Sensorgrams of lncEry-P5 binding to Wdr82 were measured by SPR technology using a Biacore 3000 instrument (GE Healthcare). The flowing-phase RNA lncEry-P5 was synthesized by in vitro transcription using a T7 RNA production system (Promega ...
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The first step was affinity purification on an MBP-binding dextrin Sepharose column (MPBTrap, GE Healthcare: 28-9187-78). The lysates were loaded twice on the column at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity IMAC was performed by batch binding the clarified supernatants with 10mL of Ni Sepharose Excel resin (GE Healthcare). After 20-30 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... Binding responses were measured following immobilization by amine coupling of antigens (37) on CM5 sensor chips (BIAcore/GE Healthcare). Purified plasma IgG samples at 200 µg/ml were flowed (2.5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Visualization of the antibody binding sites was achieved using Amersham ECL Prime Western Blotting (GE Healthcare UK Ltd, RPN2232) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: The binding kinetics of Det7T with the microorganisms were studied using a BIAcore T200 instrument (GE Healthcare, Little Chalfont, UK). Det7T was diluted (1:50 ...
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... and those containing Tdp1 were pooled and concentrated to ~0.5 mL by binding to a small volume of SP sepharose resin (GE Healthcare) and elution with 50% buffer B ...
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bioRxiv - Cell Biology 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: NAB2 binding to GST-Nedd4 and Nedd4(HECT) was characterized via SPR using the Biacore T200 instrument (GE Life Sciences). Recombinant protein was purified as described with an additional purification step using size exclusion chromatography to ensure high purity prior to use in SPR ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by performing SEC using GST binding buffer supplemented with 0.5 mM TCEP on a Superdex 200 10/300 column using an ÄKTA purifier (GE Healthcare). The fractions corresponding to the peak were pooled ...
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... We selected four proteins as molecular mass standards from the gel filtration calibration kit HMW (GE Healthcare, Uppsala, Sweden), i.e ...
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bioRxiv - Biochemistry 2019Quote: ... The size-exclusion column was calibrated using a set of standard proteins (Gel Filtration HMW Calibration Kit, GE Healthcare) in SEC buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.