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Citations for GE Life Sciences :
501 - 550 of 6737 citations for Mouse EGF Latrophilin Seven Transmembrane Domain Containing Protein 1 ELTD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer F containing 200 mM imidazole and further purified over a 1 ml ResourceS cation exchange chromatography column (GE Healthcare) using a 20 ml gradient from 100 mM to 500 mM NaCl in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Molecular Biology 2019Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Developmental Biology 2019Quote: ... or 1 ng (for other mRNAs) of mRNAs in 1× modified Barths Solution (MBS) containing 4% Ficoll-400 (17-0300-05, GE Healthcare) using a microinjector (PLI-90 ...
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bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... after the IMAC column the proteins were diluted to buffer containing 50 mM NaCl and further purified using 1 ml Resource Q cation exchange column (GE Healthcare), where they were eluted with a linear gradient of 50 mM NaCl to 1 M NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2019Quote: ... The column was calibrated with protein standards of known molecular weight and stokes’ radius (Low and High Molecular Weight Kit, GE Healthcare) according to manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were detected by fluorescence (Odyssey, Li-Cor) or revealed using the ECL Prime Western Blotting Detection Reagent kit (GE Healthcare) and images were acquired in a dark room using a G:Box (Syngene ...
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bioRxiv - Zoology 2019Quote: The midgut BBMV proteins were subjected to clean-up using the 2-D Clean-up Kit (GE Healthcare Life Sciences, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Anti-mouse (GE Healthcare) or rabbit-HRP (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% bovine calf serum (GE Healthcare SH30072.03), 2 mM L-glutamate along with penicillin and 100 μg/ml streptomycin (Wisent 609065 ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed with HRP-conjugated anti-mouse or anti-rabbit (1:5,000, GE Healthcare Bio-Sciences) secondary antibody after washing several times with 1X PBS for 45 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... followed by anti-rabbit-IgG and mouse-IgG antibodies coupled to HRP (1:2000, NA934, NA931, GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were then incubated with anti-Mouse HRP-conjugated secondary antibody (1:5000 of NA931, GE Healthcare) for 1 hour at room temperature followed by three washes with PBST ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for western blotting are as follows: α-mouse-HRP (1:1000) (GE Healthcare, Piscataway, NJ) for α-O-GlcNAc primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931 ...
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bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...