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Citations for GE Life Sciences :
701 - 750 of 1769 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and then incubated with horseradish peroxidase-linked anti-mouse IgG (1:1000) (GE Healthcare Life Sciences). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-rabbit IgG-HRP (NA934) and anti-mouse IgG-HRP (NA931) were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Genomics 2020Quote: ... The secondary antibody was horseradish peroxidase-linked anti-mouse IgG from GE Healthcare (catalog no. NA931V) and the chemiluminescent signals were visualized with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1 hour with a secondary antibody anti-Mouse (GE Healthcare, NA9310, 1:5000 dilution), washed three times with TBS-Tween 1 % solution and revealed with an ECL Western blotting detection kit (Advansta ...
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bioRxiv - Cell Biology 2020Quote: Coding sequences were amplified from plasmids encoding mouse GPR161 (BC028163; Mammalian Gene Collection [MGC]; GE Healthcare), mouse SSTR3 (gift from Kirk Mykytyn ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then detected with HRP-conjugated anti-mouse IgG (for IgM detection; GE Healthcare) or anti-rabbit IgG (for IgT detection ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were reacted with anti-rabbit (or anti-mouse) immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used for Western blot detection were: sheep anti-mouse HRP (GE Healthcare #NA931), donkey anti-rabbit HRP (GE Healthcare #NA934V).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
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bioRxiv - Biochemistry 2023Quote: ... anti-rabbit IgG-HRP (NA934V) and anti-mouse IgG-HRP (NA931V) (GE Healthcare, GENA934 and GENA931).
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bioRxiv - Genomics 2024Quote: ... SERBP1 immunoreactivity was detected with a horseradish peroxidase– conjugated anti-mouse IgG (1:5000, GE Healthcare). GAPDH immunoreactivity was detected using an anti-mouse IgG (1:6000 ...
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bioRxiv - Microbiology 2024Quote: ... for EF1-α - anti-mouse (1:10,000 in 1% milk/PBS-T, GE Healthcare, code NA931V). Following secondary incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody used in Western blotting was HRP sheep anti-mouse IgG (GE Life Sciences NA931V).
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2019Quote: ... and electrotransfered (2 h at 80 V or 16 h at 30 V and 4°C) onto polyvinylidene difluoride membrane (Amersham, GE Healthcare). The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The clarified cell lysates were incubated for 2 h on a spinning wheel at 4°C with 100µl of StrepTactin Sepharose High Performance beads (#28935599, GE Healthcare, USA). Beads were collected by centrifugation (1600 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The complex was supplied with 2mM GTP and incubated at 4°C for 1 hour prior to purification by size exclusion chromatography on a HiLoad Superdex S75 16/600 column (GE Healthcare) equilibrated in 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate made by sonication was centrifuged at 11,000 rpm for 30 min at 4◦C and the clear supernatant was purified through Ni-NTA column chromatography (GE Healthcare) following kit protocols ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by centrifugation at 40,000 rpm in a Type 45 Ti rotor for 60 minutes at 4 °C and applied onto a pre-equilibrated HisTrap HP column (GE Healthcare) in CENP-E lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoblotting was carried out by separating the immunoprecipitates on 10% polyacrylamide gels as described using a Bio-Rad Mini-Gel apparatus and transferring them electrophoretically to Hybond-C Extra membranes (GE Healthcare) using a Trans-Blot apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... The intensities of the bands of SREBP-1c and Lamin A/C in nuclear extracts were quantified with Image analysis software (ImageQuant TL, GE Healthcare). The intensities of nuclear SREBP-1c were normalized by those of corresponding Lamin A/C intensity.
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Microbiology 2022Quote: ... The plates were scored after 24 and 48 h incubation at 37 °C and imaged using ImageQuant LAS 4000 (GE Healthcare). The last time point (24 h ...
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bioRxiv - Genomics 2020Quote: Time-lapse imaging of stable RPE1 cells expressing 53BP1-eGFP and Lifeact-RFP was performed in a 37°C climate chamber attached to a Deltavision microscope system and the SoftWorx 4.9 software (Applied Precision/GE Healthcare) using a 40x 1.3 NA oil-immersion objective ...
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bioRxiv - Biophysics 2019Quote: Proteins were concentrated with appropriate centrifugal filtration devices (Amicon) and further purified by SEC at 4°C using an ÄKTAFPLC chromatography system (GE Healthcare). For crystallographic studies ...
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bioRxiv - Microbiology 2019Quote: ... Images were acquired with 200 ms exposure time every 4 minutes for at least 6 h at 37°C using Softworx (Applied Precision) software ...
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was removed by centrifugation at 10000xg for 45 min at 4°C and the supernatant was loaded onto either a 1 mL or 5 mL HisTrap FF crude column (GE Healthcare) equilibrated in Ni-1 buffer using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
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bioRxiv - Genomics 2019Quote: ZHBTc4 YPet-MD TIR1-V5 mCherry-OCT4-AID cells were plated in a 96-well plate coated for 1 hour at 37°C with 1:25 diluted StemAdhere (Primorigen Biosciences #S2071-500UG) and imaged on an IN Cell Analyzer 2200 (GE Healthcare) using the TexasRed and Brightfield channels ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Biochemistry 2020Quote: ... PDIs and their mutants were separately reduced with 10 mM DTT for 10 min at 30°C followed by DTT removal through a PD-10 column (GE Healthcare) pre-equilibrated with 50 mM Tris/HCl (pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of FN3Con-anti-VEGFR2 was measured using surface plasmon resonance with a 30 µl/min flow rate at 25 °C (BIAcore T-100, GE Healthcare). VEGFR2 domains (SiboBiological ...