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Citations for GE Life Sciences :
451 - 500 of 6158 citations for Mouse Anti Dengue Virus Envelope Protein Serotype 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated to 1 mg/ml using a vivaspin 20 (GE Healthcare) and store at −80 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-anti rabbit secondary (GE Healthcare; 1:5000) and HRP-anti mouse secondary (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-rabbit (NA934, GE Healthcare, 1:10000) and sheep anti-mouse (NA931 ...
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bioRxiv - Cell Biology 2021Quote: ... and donkey anti-rabbit (GE healthcare; 1:5000).
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-rabbit (#NA9340V, GE Healthcare, 1:4000). Membranes were washed after each incubation step three times for 5 min in TBS-T buffer (25 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-rabbit (NA9340; GE Healthcare; 1:25,000). Protein extraction was performed using lysis Buffer (20 mM Tris (pH 8) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit secondary antibody (GE healthcare,1:10000). Membranes were developed by the Odyssey Fc Imaging system (LI-COR Bioscience).
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bioRxiv - Cancer Biology 2021Quote: ... Immunocomplexes were detected using sheep anti-mouse or donkey anti-rabbit immunoglobulin secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare Europe GmbH) and visualized by enhanced chemiluminescence (Super Signal West Pico ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed for 3x 5 minutes with TBST and incubated for 1 hour at RT with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, Jackson ImmunoResearch 111-035-144 or sheep anti-mouse IgG, GE Healthcare NA931V) diluted 1:5000 in 5% milk / TBST ...
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bioRxiv - Genomics 2020Quote: ... The secondary antibody was horseradish peroxidase-linked anti-mouse IgG from GE Healthcare (catalog no. NA931V) and the chemiluminescent signals were visualized with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then detected with HRP-conjugated anti-mouse IgG (for IgM detection; GE Healthcare) or anti-rabbit IgG (for IgT detection ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used for Western blot detection were: sheep anti-mouse HRP (GE Healthcare #NA931), donkey anti-rabbit HRP (GE Healthcare #NA934V).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody used in Western blotting was HRP sheep anti-mouse IgG (GE Life Sciences NA931V).
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Genetics 2020Quote: ... Proteins of interest were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (GE Healthcare). Peroxidase substrate was provided by ECL Western Blotting Substrate kit (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... Anti-TNFα IgG1 was purified on Protein A HiTrapHP columns (GE Healthcare Life Sciences, Little Chalfront, UK) using Akta-prime plus (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... Anti-DEC205-OVA was purified from the media using a HiTrap Protein G HP column (GE Healthcare) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The ITC protein sample p52 (1-398) went through desalting column (HiTrap desalting, GE Healthcare) to freshly made ITC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2020Quote: ... using multiple runs over a 1 ml Protein-A HiTrap column (GE Healthcare Life Sciences) on an ÄKTA FPLC (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was loaded on to a 1 ml Mono S column (GE Healthcare), eluted with a 20-bed volume linear gradient from 0 to 500 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Genomics 2020Quote: ... diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare) diluted in TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... Horseradish peroxidase (HRP)-linked secondary antibodies included anti-mouse IgG (sheep polyclonal, NA931: GE Healthcare, Buckinghamshire, UK), anti-rabbit IgG (sheep polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with anti-mouse HRP-conjugated secondary antibody from GE Healthcare (Cat. NA9311ML, Chicago, USA). The bands were analyzed by an Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies and stains used were: IgG peroxidase-conjugated anti-mouse IgG from donkey (GE Healthcare, NA931), anti-rabbit IgG from donkey (GE Healthcare ...