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Citations for GE Life Sciences :
201 - 250 of 4014 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... diluted with 4 mL 0.5% Triton X-100 in PBS (PBS-T) and incubated with prepared glutathione sepharose beads (GE Healthcare) in a gravity column for 3 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... peak fractions were diluted to 100 mM NaCl and loaded onto a 5 ml HiTrap Q XL column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2021Quote: ... and eluted with PBS at a rate of 0.5 ml/min on an AKTA Explorer 100 FPLC Workstation (GE Healthcare). The protein peaks observed were collected in Eppendorf tubes in a volume between 0.5 and 1 ml ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... purified TRAPPIII and miniTRAPPIII (~100 μl at 0.5 mg/ml) were resolved on a Superose 6 10/300 column (GE Healthcare) in Buffer B ...
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bioRxiv - Biochemistry 2022Quote: Samples of the different complexes were prepared at 10 μM and 100 μl were injected onto a 2.4 mL Superdex 200 Increase gel filtration column (GE Healthcare) pre-equilibrated in 100 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... were flowed through with a concentration ranging from 0.05 to 100 nM and the data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... The eluate was concentrated to 2 mL and then loaded onto a Superdex 200 column (GE Healthcare, Cat. #10034543) for size exclusion chromatography (S200 buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP) and applied it onto a 1 ml HiTrap SP HP Sepharose column (GE Healthcare). Cas9 was eluted using a linear gradient from 0-100% buffer B (20 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded at low speed (0.2 ml/min) at 4°C onto a HisTrap-HP 1 mL affinity column (GE Healthcare) equilibrated with buffer A (20 mM NaPi pH 7.7 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2021Quote: ... iMAC samples were thawed on ice and loaded into a purification column packed with 10 mL of Sephacryl S-100 (GE Healthcare) equilibrated with 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted using a gradient increasing the salt concentration to 500 mM KCl over 100 mL while collecting fractions (AEKTA prime FPLC system, GE Healthcare). Fractions were analyzed by SDS-PAGE and those containing pure full-length TwinStrep-UPF1 were collected ...
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bioRxiv - Biophysics 2021Quote: ... The pooled fractions were dialyzed against buffer A + 100 mM NaCl before loading onto a 5 mL Hitrap Heparin column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
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bioRxiv - Cancer Biology 2022Quote: Primary human AML specimens and normal MPB samples were resuspended at about 100-200 e6 cells per ml in freezing media composed of 50% FBS (GE Healthcare), 10% DMSO (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... was then centrifuged at 21,000 ×g at 4 °C for 10 min and 100 µL of the supernatant was loaded on to a Superdex 200 column (25 mL, GE Healthcare) equilibrated with the Buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... the soluble lysate was loaded onto 1 mL Nickel beads (GE healthcare). The column was washed with lysis buffer containing 15 mM imidazole and his-m6A-Tracer was eluted by 250 mM imidazole in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction was loaded on 1 mL Nickel beads (GE Healthcare). Beads were washed with lysis buffer containing 20 mM imidazole before protein was eluted by 250 mM imidazol in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was loaded onto 1 mL Ni-affinity column (GE healthcare). After washing with the same buffer and the storage buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... the sample was loaded on a 1 mL MonoQ column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... a HiTrap Q FF anion exchange chromatography column (1 mL, GE Healthcare) was used on an ÄKTA-FPLC (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... and loaded onto 1 mL MBP Trap™ HP column (GE Healthcare) following equilibration with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The elution was applied to a 1 mL StrepTactin column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and applied to a HisTrap column (1 ml bed volume, GE Healthcare) at a drop rate of 500 μl/min at 4 °C for o/n ...
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bioRxiv - Plant Biology 2021Quote: ... using an Äkta explorer system (GE Healthcare, loading speed 1 ml/min) with buffers A (50 mM MES ...
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bioRxiv - Synthetic Biology 2021Quote: ... HP columns (1 mL) (both GE Healthcare Life Sciences, Little Chalfont, UK). A buffer exchange was carried out to remove excess imidazole using an Amicon® Ultra-15 Centrifugal Filter Unit – 3 kDa MW cutoff (UFC900308 ...
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bioRxiv - Microbiology 2021Quote: ... StrepII-CERT was captured using 1 mL StrepTrap HP column (GE Healthcare) that had been pre-equilibrated in wash buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and loaded onto a 1-mL HiTrap IMAC-HP column (GE Healthcare), which was pre-equilibrated with lysis buffer containing 0.1% dodecyl maltoside ...
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bioRxiv - Molecular Biology 2022Quote: ... a HiTrap Q FF anion exchange chromatography column (1 ml, GE Healthcare) was used on an ÄKTA-FPLC (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
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bioRxiv - Biophysics 2020Quote: ... we load the solution on a 1 mL MonoQ column (GE Healthcare) using an FPLC system (Äkta Explorer) ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded on 1 mL HiTrap Heparin HP (GE Healthcare, Cat# 17040601) equilibrated with buffer C (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1 ml HisTrap column (GE Healthcare) previously equilibrated with Buffer H ...