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Citations for GE Life Sciences :
351 - 400 of 1032 citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... inoculum was removed and cells were overlaid with DMEM + 10% fetal bovine serum (GE Healthcare Life Science, Piscataway, NJ, USA). After 1 day ...
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bioRxiv - Microbiology 2021Quote: ... The second antibodies used for ELISA were as follows: HRP-linked anti-whole rabbit IgG donkey serum (GE Healthcare, #NA934) for detection of the positive control ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgM were separated from serum by affinity chromatography using HiTrap Protein G High Performance column (Cytiva, GE Healthcare) and CaptureSelect IgM Affinity Matrix (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... cancer cell lines were grown in Dulbecco’s modified Eagle’s medium (GE Healthcare Life Sciences Logan, UT) supplemented with 10% fetal bovine serum (10% FBS-DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... HepG2 and HEK293 cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... HeLa Tet-off cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% tetracycline-free FBS (Wisent ...
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bioRxiv - Molecular Biology 2022Quote: ... and white blood cells from plasma were isolated with Ficoll-Paque™ PLUS medium (GE healthcare). Thereafter ...
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bioRxiv - Immunology 2020Quote: ... Secreted ACE2 PD was isolated from conditioned cell culture medium using Nickel Excel resin (GE Healthcare) followed by gel filtration chromatography on a Superdex200 10/30 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were grown in HyClone Dulbecco’s Modified Eagle Medium with high glucose (GE Healthcare Life Sciences) supplemented with L-Glutamine (WISENT Inc) ...
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bioRxiv - Cell Biology 2021Quote: ... and subjected to size-exclusion chromatography using Sephadex G-25 (Medium) columns (GE Healthcare Life Science) to remove external ADP or AMP ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were maintained in HyClone™ Dulbecco’s High Glucose Modified Eagles Medium (DMEM, GE Healthcare), HyClone™ McCoy’s 5A (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The medium was passed over a 5 ml HiTrap Chelating column charged with nickel (GE healthcare), washed with 30 ml of buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... PD-10 desalting column containing Sephadex G-25 medium (#GE17-0851-01) was from GE healthcare, and Amicon Ultra-4 centrifugal filter devices ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The oligomers were prepared by diluting the DMSO stock with serum-free and phenol red-free DMEM (GE Life Sciences #SH30284.01) and incubating at 4°C for 24 hours without shaking ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Neuroscience 2021Quote: ... or 90 V (secreted fraction and serum) at room temperature (RT) and electro-transferred to 0.45 µm nitrocellulose membranes (Amersham, GE Healthcare, Germany) for 1h30-2h at 0.5 A at 4°C ...
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Immunology 2021Quote: ... moDC were gently washed twice with T cell expansion medium (RPMI supplemented with HyClone FBS [GE Healthcare, Chicago ...
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bioRxiv - Cancer Biology 2020Quote: ... All breast cancer cell lines were maintained in Dulbecco’s Modification of Eagle’s Medium (DMEM, GE Life Sciences) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... while HCT-15 and HL-60 cells were grown in RPMI medium (Hyclone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (TianJin Haoyang Biological Products Technology Co. ...
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bioRxiv - Microbiology 2022Quote: ... USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Cat# SH30022.01, GE Healthcare Life, Pittsburgh, PA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... All other cell lines were grown in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences, Logan, UT) with 10% fetal bovine serum (10% FBS–DMEM) ...
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bioRxiv - Cancer Biology 2024Quote: ... and PC3M cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone, GE Healthcare Life Sciences, Logan, UT) supplemented with 10% fetal bovine serum (HyClone ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... Prior to infection HFFs were grown to confluency in DME supplemented with 10% v/v cosmic calf serum (GE Healthcare, New Zealand) (D10) ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were re-hydrated using PBS-0.1% Triton X-100 (PBT) and then blocked for 20 minutes using 20% Normal Goat Serum (NGS) (GE Healthcare, Amersham RPN410) in PBT ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... ECL chemiluminescent kit (RPN2132, GE Healthcare) was used for detection according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The ECL Prime Kit (GE Healthcare) was used for HRP detection.
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from conditioned medium by cation exchange chromatography on a SP sepharose column (GE healthcare) in 50 mM Tris ...