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Citations for GE Life Sciences :
151 - 200 of 4290 citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS; GE Healthcare Bio-Sciences, Chicago, IL), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Biophysics 2019Quote: ... The 12xHis-tag was removed by overnight digestion with 3C protease (GE Healthcare Life Sciences) followed by a purification step with Ni-NTA agarose (QIAGEN ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... that had encoded an N-terminal His6-tag followed by a PreScission protease (GE Healthcare) cleavage site ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes from the lungs of influenza virus infected mice were enriched using Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient and stained with H-2Db restricted tetramers conjugated to fluorophore R-phycoerythrin (PE ...
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bioRxiv - Immunology 2019Quote: ... His10-tag free CD80 ectodomain was purified with a StrepTrap HP column (GE Healthcare, catalog 28907547) in 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... to remove the GST tag and further purified by gel filtration (Superose 6, 10/30; GE Healthcare). For cryo-EM investigation ...
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bioRxiv - Molecular Biology 2019Quote: ... The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare) equilibrated in BRB80 buffer (80 mM PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] pH 6.8 ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The tag-free Fv fragment was concentrated and loaded onto a HiLoad16/60 Superdex75 column (GE Healthcare) equilibrated with TBS buffer ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Plant Biology 2023Quote: ... Each of the His-tag-fused LssaCA muteins was bound to Ni Sepharose (GE Healthcare, Uppsala, Sweden), then the GST-NP protein or the GST control protein was added to the beads ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Immunology 2023Quote: ... and Fc using size exclusion chromatragraphy with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated using 100 kDa Amicon concentrators ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Molecular Biology 2019Quote: ... Ni-NTA re-capture of the cleaved tag and size-exclusion chromatography (Superdex 75 16/60, GE Healthcare). Timeless 883-947 (DBD C-term ...
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bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Cell Biology 2020Quote: ... Scd1 (aa 10-872) and Rga4 (aa 389-1150) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare), expressed in BL21 (DE3 or Rosetta ...
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bioRxiv - Biochemistry 2022Quote: ... The tag cleaved Ub was further purified by size exclusion chromatography through a Superdex 75 column (GE Healthcare) in buffer A.
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bioRxiv - Biochemistry 2023Quote: ... The His-tag was separated from DBD on an S75 10/300 GE gel filtration column (GE healthcare) equilibrated with 10 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Microbiology 2021Quote: ... 100μg/ml of streptomycin and 5% fetal calf serum (FCS; HyClone, GE Healthcare, South Logan, UT, U.S.A.) at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...