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Citations for GE Life Sciences :
601 - 650 of 659 citations for Lipoteichoic Acid LTA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Total RNA from brain tissues or culture cells were extracted using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare Life Sciences) for reverse transcription with the High-Capacity cDNA Reverse Transcription Kits (ABI Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s instructions and circular DNA was enriched by rolling circle amplification (RCA) with TempliPhi™ amplification kit (GE Healthcare, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were further washed three times for five minutes in PBS-T before being visualised using ECL kit (GE Healthcare Cat. #RPN2232) and ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein samples were subjected to immunoblotting for target proteins and immunoreactive bands were visualized using the Amersham ECL western blotting detection kit according to the manufacturer’s protocol (GE Healthcare, Piscataway, NJ). Antibodies for GAPDH or Cyclophilin B were used as loading controls.
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bioRxiv - Genomics 2019Quote: ... and whole genome amplification was achieved by multiple displacement amplification with Phi29 DNA polymerase and the Illustra GenomiPhi v.2 kit (GE Healthcare, 25660032). Amplified genomic DNA (70 ng ...
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bioRxiv - Molecular Biology 2019Quote: ... random hexamer primers were used following the manufacturer’s instructions for the GenomiPhi V2 DNA Amplification Kit (catalogue # 45-001-221, GE Healthcare Life Sciences).
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bioRxiv - Genetics 2020Quote: We performed WGA on each F2 gDNA cercarial sample using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, USA) according to Le Clec’h et al ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were developed using the ECL Prime kit (Cytiva, Marlborough, MA) and imaged using Amersham Imager 600 (GE Healthcare, Amersham, Buckinghamshire, UK). Fractionation was repeated with 3 independent sets of cultures and one set of immunoblots is presented as a representative.
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bioRxiv - Microbiology 2020Quote: ... extracted DNA was subjected to multiple displacement amplification (MDA) using the illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA from 30–50 light-treated or dark control embryos was extracted using the illustra™ RNAspin Mini kit (GE Healthcare). 1 μg total RNA was used for reverse transcription with qScript XLT cDNA SuperMix (Quantabio) ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Genomics 2021Quote: Single-cell WGA was done on 10~17 μm or 17~25 μm C1 DNA-Seq IFC (Fluidigm, 100-5763 or 100-5764) via multiple displacement amplification method using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, 25660031). The WGA products from single cells were mixed according to their tissue origin ...
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bioRxiv - Molecular Biology 2022Quote: ... The probes (PCR amplified from gDNA or binary plasmid using the oligos in Supplemental Table S3) were internally labelled using [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and hybridisation and washes were performed as described ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
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bioRxiv - Microbiology 2022Quote: ... whole genome amplification was carried out using the multiple displacement amplification technique with the Genomiphi V2 DNA Amplification Kit (GE Healthcare, USA). This approach has been widely used previously in various works [18–20] ...
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bioRxiv - Microbiology 2023Quote: ... All genes used in the curves were purified with the IllustraTM GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) for better efficiency of the standard curves ...
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bioRxiv - Pathology 2023Quote: The circular DNA genomes of MSV were first amplified by rolling circle amplification (RCA) according to the manufacturer’s protocol (TempliPhiTM kit, GE Healthcare, Munich, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized using an ECL kit (Ez WestLumi plus, ATTO, Tokyo, Japan) via chemiluminescence (ImageQuant LAS 4000 mini, GE Healthcare, USA) and quantified using NIH ImageJ software (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were further washed 3x with TBST and once with TBS before visualization of the resulting bands with ECL Advanced Western Blotting Detection Kit (GE Healthcare, #RPN2135) in an Amersham Imager 600 (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... The two fragments were fused by PCR to obtain a product corresponding to the 783 bp coding sequence of TMEM70 transcript variant 1 that was cloned into pMOSblue vector using the Blunt-Ended PCR Cloning Kit (GE Healthcare Life Sciences) to generate the pMOS-TMEM70 plasmid ...
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bioRxiv - Physiology 2020Quote: Distinct bands of correct size were cut from 1.5% agarose gel and isolated using illustra GFX PCR and Gel Band Purification Kit (GE Healthcare, Chicago, IL, USA), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed by agarose gel electrophoresis with ethidium bromide staining and purified using the Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, Chicago, IL). Reactions (10 μL ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Synthetic Biology 2022Quote: CM3 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Immunology 2020Quote: CM5 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Five microliters of each DNA sample were amplified in a 50-ul reaction using an Illustra GenomiPhi DNA V3 DNA amplification kit (GE Healthcare, United Kingdom).
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bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
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bioRxiv - Plant Biology 2022Quote: Leaf samples from ino-4 and ino-4 i20 plants were separately pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the provider’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was processed for amplification of complete genome of the virus by rolling circle amplification (RCA) method using TempliPhi illustra amplification kit (GE Healthcare, Piscataway, NJ). The resulting RCA product (2 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and digested using the Macs Neural tissue Dissociation kit (Miltenyl Biotec) and subsequent percoll gradients (30% percoll [GE healthcare], 10% fetal bovine serum) was used to purify myeloid cells and incubated to antibodies against Cd11b and CD45 ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Cancer Biology 2021Quote: ... Whole genome DNA was amplified using 1 μl polymerase enzyme from the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Cat# 25-6600-32) and 9 μl of sample with the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Chemiluminescence detection was performed using the ECL Prime Western Blotting kit (GE-healthcare, RPN2232) and developed on a ImageQuant LAS 4000 (GE healthcare, 28-9558-10). Secondary antibodies for infrared fluorescence detection were donkey anti-rabbit-IRDye® 800CW (Rockland ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of each field sample was subject to whole genome amplification using the illustra Genomiphi V2 DNA amplification kit (GE Healthcare Life Sciences, Australia) for 2 hours at 30°C followed by heat inactivation at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were developed using the WesternBright™ Quantum kit (K-12042-D10, Avansta) and visualized using an Amersham Imager 680 (GE Healthcare Life Sciences) system.
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5) was immobilized on the surface of a CM5 sensor chip using a Amine Coupling Kit (GE Healthcare Bio-Sciences AB, Sweden). VHHs were serial 4-fold diluted from 417 nM to 0 nM and flowed over the captured RBD surface at 15 μl/min for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The samples were transferred to a positively charged membrane using southern blot protocol from the Whatman Nytran SuPerCharge TurboBlotter kit (Whatman GE Healthcare, Little Chalfont, UK). Air dry the membrane ...
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bioRxiv - Microbiology 2022Quote: ... Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The remainder of the extraction was performed with a modified version of the Illustra RNAspin mini RNA isolation kit (GE Healthcare, #25-0500-71) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...