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Citations for GE Life Sciences :
1 - 50 of 5229 citations for Leucine Rich Repeats And Immunoglobulin Like Domains Protein 3 LRIG3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Plant Biology 2021Quote: ... The peroxidase-conjugated donkey anti-rabbit immunoglobulin antibody (GE Healthcare) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins or domains were immobilized on CM5 chip (GE Healthcare, USA) at response unit 2914.6 ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies were used together with horseradish peroxidase-conjugated α-mouse immunoglobulin G (1:5000, GE Healthcare) as secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked anti-rabbit immunoglobulin G (both GE Healthcare Bio-Sciences, Pittsburgh, PA). Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse immunoglobulin (Ig)-G horseradish peroxidase (HRP)-linked secondary antibody (Amersham catalogue number NA931, GE Healthcare, Buckinghamshire UK) diluted 1:4000 was incubated at RT for 2 hours before final washes and 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Cell Biology 2020Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked antirabbit immunoglobulin G (both Cytiva, formerly known as GE Healthcare Bio-Sciences NA931 and NA934), Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Genetics 2022Quote: ... or goat-developed anti-mouse immunoglobulin G (GE Healthcare, NA931). Membranes were developed with ECL+ and signals were obtained using ChemiDoc XRS+ and analyzed by Image Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) or donkey anti-goat immunoglobulin (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Cell Biology 2022Quote: ... or sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) and the enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
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bioRxiv - Microbiology 2024Quote: ... together with horseradish peroxidase conjugated sheep anti-mouse immunoglobulin G (GE Healthcare) as a secondary antibody (dilution 1:2000) ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunocomplexes were detected using sheep anti-mouse or donkey anti-rabbit immunoglobulin secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare Europe GmbH) and visualized by enhanced chemiluminescence (Super Signal West Pico ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Microbiology 2021Quote: ... and 100 µl peroxidase-conjugated goat anti-mouse immunoglobulin G (IgG) (GE Healthcare), IgG1 ...
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bioRxiv - Immunology 2020Quote: ... Purification of the immunoglobulins was carried out by Akta Start System (GE Healthcare) using protein G column ...
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bioRxiv - Cell Biology 2023Quote: ... then reacted with goat anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... followed by reaction with anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution and visualized by ECL ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Biophysics 2019Quote: ... After cleavage of the 6xHis-lipoyl domain with thrombin (GE Healthcare), the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were incubated with immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) on a rotating wheel at 4 °C for 90 min ...
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bioRxiv - Microbiology 2022Quote: ... were used together with horseradish peroxidase conjugated sheep anti-mouse immunoglobulin G (GE Healthcare) as a secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was purified using a HisTrap excel column (GE Healthcare). The filtrated media were loaded into the HisTrap excel column ...
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bioRxiv - Biochemistry 2023Quote: ... for the PWWP domain or a HiTrap Q HP column (GE Healthcare) for the ADD domain ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 μg of GST-FG-repeat were incubated with 50 μL Glutathione Sepharose 4B beads (GE healthcare) at 4°C for 3 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...