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Citations for GE Life Sciences :
1 - 50 of 104 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The His-tag free MUC5AC-D3 variants were loaded onto a HiPrep Q HP 16/10 anion exchange chromatography column (GE Healthcare) and eluted in a 0-500 mM NaCl gradient in 20 mM Tris pH 8.
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bioRxiv - Biophysics 2021Quote: ... aSyn was buffer exchanged into 5% D2O and 95% H2O using PD10 desalting columns (GE Healthcare). CsI and NaCl were added to a final concentration of 150 mM and 1.5 M just before performing the experiments.
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bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
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bioRxiv - Biophysics 2021Quote: ... 150 mM CsI or 1.5 M CsI in H2O or D2O prior to performing experiments using PD10 desalting columns (GE Healthcare). 10 μM freshly made ThT (abcam ...
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bioRxiv - Microbiology 2021Quote: ... The gel was rinsed with double distilled H2O and scanned at the TAMRA channel on a Typhoon Scanner (GE Healthcare). In an alternative procedure ...
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bioRxiv - Plant Biology 2024Quote: ... gels were placed in containers with H2O and scanned directly using a Typhoon fluorescence scanner (Model FLA9500, GE healthcare, USA) with the following settings ...
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bioRxiv - Immunology 2023Quote: ... using the 18O(p,n)18F nuclear reaction fluorine-18 was produced as [18F]fluoride by proton irradiation of [18O]H2O (Rotem, Leipzig, Germany) at the Tübingen PETtrace cyclotron (GE Healthcare, Uppsala, Sweden).
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bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μl of pre-equilibrated protein L beads (Capto™ L resin, GE Healthcare Cat no. 17547802 Cat. no. 17547803) was added and reaction was incubated for 90 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM L-Glutamine (GE Life Sciences #SH30852.01), 100 units/mL Penicillin and 100 μg/mL Streptomycin (Corning #30-001-CI ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with 4.5 mM L-glutamine (GE Life Sciences) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Molecular Biology 2020Quote: ... the AKTA pure L chromatographic system (GE Healthcare) was used ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Molecular Biology 2020Quote: ... coli 55244 cells (ATCC) and purified using Protein L (GE Healthcare) gravity flow affinity chromatography as published previously29 ...
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies α-mouse IgG (H&L)-HRP (GE healthcare #NXA931), 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934) ...
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bioRxiv - Microbiology 2020Quote: ... 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934), 1:5000 ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Bioengineering 2023Quote: ... The rhEPO was enriched onto 0.40 L Capto blue (GE Healthcare, USA) column with AKTA Avant 150 system (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Cell Biology 2021Quote: ... (w/o L-glutamine and phenol red, GE Healthcare Bio-Sciences, Pittsburgh, PA). After removal of the medulla ...
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bioRxiv - Bioengineering 2023Quote: ... The retentate was loaded onto 0.25 L Q Sepharose HP (GE Healthcare, USA) column to bind desired isoforms ...
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bioRxiv - Cell Biology 2024Quote: ... or goat α-mouse IgG (H&L)-HRP (1:10,000, GE healthcare #NXA931), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... the L proteins were further purified by size-exclusion chromatography (Superose 6, GE Healthcare) in buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... Knockdowns of CDK1 was performed by using SMARTpool reagents (L-003224-00-0005; GE Healthcare) and ON-TARGETplus nontargeting siRNA (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... The elute from Q Sepharose was loaded on to 0.20 L source 15RPC (GE Healthcare, USA) column to separate non-glycosylated and glycosylated rhEPO ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Heeseon An) were maintained in McCoy’s 5A (modified) medium with L-Glutamine (#SH30200.01, GE Healthcare, USA) medium supplemented with a 10% final concentration of fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were treated with Chemi-Lumi One L (Nacalai Tesque) or ECL prime (GE Healthcare), and the signal was detected by Amercham ImageQuant 800 (Cytiva).
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Biophysics 2020Quote: The transducin heterotrimer was isolated from the rod outer segment of bovine retina (W L Lawson Company) and Gβ1γ1 was separated from Gαt with Blue Sepharose 6 Fast Flow (GE Healthcare) as described(21) ...
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bioRxiv - Biophysics 2022Quote: ... These cells were cultured in RPMI 1640 with 2.05mM L-glutamine (GE Healthcare Bio-Sciences Corp., MA, USA) supplemented by 5% v/v fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine, GE Healthcare Life Sciences SH30065.02) supplemented with 50 U/ml Penicillin-Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 40 mM HEPES pH 7.4 (4 °C) ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...