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Citations for GE Life Sciences :
251 - 293 of 293 citations for Isotachysterol 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... The culture medium containing pseudotyped particles was collected after 48 hr and concentrated 3 times using Vivaspin columns with 100 kDa cutoff (GE Healthcare, #28932363) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Before revelation membranes were washed again 3 times in TBS-T for 5min and then incubated in ECL™ Western Blotting Detection Reagent (RPN2209, GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The next day the protein was dialyzed for an additional 3 hours in fresh storage buffer and subsequently applied to a Superdex 200 10/300 GL (GE Life Sciences) equilibrated in the same buffer ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science) and 3.2% acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... slice thicknesses of 1.5 and 3 mm) with or without intravenous iodixanol contrast medium (320 mg/mL; GE Healthcare, Little Chalfont, UK) and evaluated for IVCF radiopacity ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Molecular Biology 2023Quote: ... the NbaAgo samples (3 mg/ml in 33 μl) were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare, USA) operated on a ProStar335 system and connected to a tandem of Prostar 335 detector (Varian ...
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bioRxiv - Microbiology 2023Quote: Codon-adapted genes (see above) of vhemO and vpcyA/X from the CB_2 phage were cloned in pGEX-6P-3 (GE Healthcare Life Sciences), resulting in a tac promoter-driven expression of a glutathione-S-transferase fusion protein with the protein of interest ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... albicans Phr1 and Pga4 gene without the sequence encoding signal peptide and the GPI anchor were constructed using primers listed in Supplementary Table 3 and cloned into the bacterial expression vector pGEX6P1 (GE healthcare, Stockholm, Sweden). Plasmids were then transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... SepFΔML,K125E/F131A were covalently immobilized on 3 independent flow-cells of an NTA sensorchips as previously described 64 (GE Healthcare Life Sciences). The final immobilization densities of SepF variants ranged from 3000 to 4000 resonance units (RU ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
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bioRxiv - Biochemistry 2020Quote: ... The fractions were collected and concentrated to a final concentration of 700 µM using Vivaspin sample concentrators (GE Healthcare; cut-off: 3 kDa). The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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bioRxiv - Bioengineering 2023Quote: All experiments were performed on a 3 Tesla whole-body GE Premier system equipped with a 21-channel receive head coil (GE Healthcare, Milwaukee, WI). We used fluoroptic probes for the gold standard temperature measurement with a precision of 0.1 °C and a temporal resolution of 1 second (STB Medical ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Positive RDT samples were then used to create dried blood spots (DBS) on 3 mm thick Whatman® filter paper (Whatman, GE Healthcare, UK). The DBS was dried at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Molecular Biology 2019Quote: A paper punch was used to make 3 mm diameter circles of Whatman™ Grade 1 180 μm thick cellulose filter paper (GE Healthcare Life Sciences), which were stored in sterile disposable petri dishes ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...