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Citations for GE Life Sciences :
451 - 500 of 2279 citations for Inositol polyphosphate 1 phosphatase INPP1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Systems Biology 2023Quote: ... The lysates were clarified by centrifugation for 1h at 4ºC at 12000 rpm and loaded on a His GraviTrap™ column (GE Healthcare). After washing with 10 mL of washing buffer (40 mM sodium phosphate ...
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bioRxiv - Immunology 2022Quote: ... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
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bioRxiv - Plant Biology 2022Quote: ... radioactive signals from His-PIN1L were detected on the dried gel by the Typhoon imaging system (GE Healthcare TYPHOON FLA 9500).
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: Protein purification included two steps of immobilized metal-affinity chromatography separated by a thrombin-catalyzed His-tag cleavage and thrombin removal on a heparin column (GE Healthcare), hydrophobic interaction chromatography (HIC ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Bioengineering 2023Quote: ... coli lysates by immobilized metal affinity chromatography (IMAC) using a BioRad NGC FPLC equipped with a His-trap FF Ni2+ − NTA column (GE Healthcare). Columns were regenerated fresh with nickel prior to purification of each sample ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved GST-tag and bZip construct were separated by gel filtration chromatography using Hi-Load 16/600 Superdex 200 pg (GE Healthcare) on AKTA pure in a 20 mM MES pH 6.0 ...
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bioRxiv - Genetics 2023Quote: ... RAD51 without the His-SUMO tag was then recovered into the flow thru and directly loaded onto a HiTrap heparin column (GE Healthcare). The column was washed with W2 buffer and then a 0.1-1M NaCl gradient was applied ...
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE Healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... and deletion mutant preps were first diluted tenfold with chromatography buffer before being loaded through a Hi Trap Q XL column (GE Healthcare). A linear gradient of sodium chloride from 0 to 1 M was used to purify the chaperonins where each eluted at a conductivity of 5.5 mS/cm ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The middle two layers were removed for subsequent desalting (using five connected Hi-Trap desalting columns (GE Healthcare, Chicago, IL, USA) on an AKTA Pure FPLC system) ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized Glby was purified using the His-Tag via Ni-NTA based metal affinity chromatography using an AKTA FPLC (GE Healthcare, USA). The protein sample was then washed with at least 5 column volumes in washing buffer (identical to sonication buffer except for sarkosyl and PMSF) ...
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bioRxiv - Molecular Biology 2022Quote: ... Hexahistidine-tagged proteins in the soluble fraction were affinity purified using a nickel-charged His-trap immobilized metal affinity chromatography (IMAC) column (GE Healthcare, UK). Washing was carried out with lysis buffer and recombinant proteins were eluted with the same buffer containing 500 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... histones were further purified by tandem ion exchange chromatography using HiTrap Q and Hi Trap SP anion and cation exchange columns (GE Life Sciences). Histone-containing fractions were pooled ...
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bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The lysed cells were centrifuged and the supernatant filtered and applied to a single 1ml His-Trap FF (GE Healthcare Life Sciences, WA) column that was dedicated to each ARF ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.