Labshake search
Citations for GE Life Sciences :
651 - 700 of 1096 citations for Ig lambda constant 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...
-
bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... monocyte or a macrophage were isolated directly from human whole blood using Ficoll-Paque (Premium, GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
-
bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
-
bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
-
bioRxiv - Biochemistry 2020Quote: ... and 2 mM dithiothreitol) and loaded onto Superdex S200 column (GE Healthcare) The column was equilibrated and the protein was eluted with buffer C (20 mM Tris-HCl pH 8 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences) for expression in BL21 bacteria ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.2 using a 2×5ml HiTrap desalting column (GE Life Sciences) and concentrated to 10 mg/mL in a centrifugal concentrator ...
-
bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) was passed through HisTrapTM FF (GE Healthcare Life Sciences) column ...
-
bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... and applied to tandem fast-flow Ni+2-NTA HisTrap (GE Healthcare) columns ...
-
bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
-
bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 2 mL Ni-NTA resin (GE Healthcare), equilibrated with buffer A (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... RMI1/2 was further purified using a Superdex 200 Increase (GE Healthcare) in buffer I ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
-
bioRxiv - Cell Biology 2022Quote: ... Images for Figure 2 were obtained using a DeltaVision Elite (Applied Precision) maintained at 37°C and 5% CO2 equipped with a 40x and 63x PLANApo S lens (Olympus ...
-
bioRxiv - Immunology 2023Quote: ... C4BPα1-2 was applied to a HiTrap Q HP column (GE Healthcare) and eluted using a 0-1 M NaCl gradient in 50 mM Tris ...
-
bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation of peripheral blood from healthy human donors through a Ficoll-Paque PLUS (GE Healthcare) gradient ...
-
bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
-
bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
-
bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
-
bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
-
bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
-
bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
-
bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
-
bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
-
bioRxiv - Biochemistry 2023Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) and human ZIP8 (GenBank access number: BC012125) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
-
bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
-
bioRxiv - Cell Biology 2023Quote: ... was constructed by inserting the cDNA for human SSH1 (869–1049) into the BamHI and NotI sites of pGEX-4T (GE Healthcare). E ...
-
bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
-
bioRxiv - Microbiology 2021Quote: ... and applied to column with 2 ml of Ni2+-NTA-agarose (GE Healthcare) equilibrated with wash buffer (50 mM NaH2PO4 ...
-
bioRxiv - Biochemistry 2022Quote: ... the lysate was loaded on a 2×5ml HisTrap HP column (GE Healthcare) pre-equilibrated with HisBind A ...
-
bioRxiv - Molecular Biology 2019Quote: ... α-rabbit HRP-linked F(ab’)2 (GE Life Sciences NA9340; 1:5000) and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
-
bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
-
bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
-
bioRxiv - Physiology 2022Quote: ... Fractions were collected in a Whatman 2 ml 96-well plate (GE Healthcare) using a horizontal snaking pattern and were concatenated into 24 final fractions for proteomic analysis ...