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Citations for GE Life Sciences :
401 - 450 of 885 citations for IL 9 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Protein samples were separated by standard Laemli SDS-PAGE on 9% Tris-Glycine gels and transferred onto nitrocellulose membrane (GE Healthcare Bio-Sciences). PhosTag gels were prepared by adding 200 μM PhosTag™ (WAKO chemicals ...
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bioRxiv - Physiology 2020Quote: ... transthoracic echocardiography was performed for cardiac examination using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France) as described in the supplemental methods herein ...
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bioRxiv - Plant Biology 2022Quote: ... The sterilized seeds were transferred to 9 cm petri dishes filled with moistened Whatman Glass Microfiber Filters GF/A (GE Healthcare, Little Chalfont) and incubated at 25 °C for 4-7 days in dark conditions ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Neuroscience 2020Quote: ... blots were visualized using the ECL western blotting detection reagent (GE Healthcare, IL, US; Cat. No. RPN2106) on an electrochemiluminescence10 instrument (Amersham Biosciences ...
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bioRxiv - Physiology 2019Quote: ... whole-blood samples (20 µL) were transferred onto Whatman FTA® microcards (GE Healthcare, Chicago, IL, USA). The samples were allowed to dry at room temperature and stored separately in plastic bags at −20°C ...
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bioRxiv - Biochemistry 2019Quote: ... Structured illumniation microscopy was performed using a Deltavision OMX-V4 Blaze (GE Healthcare, Chicago, IL, United States) setup equipped with 4 sCMOS (PCO ...
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bioRxiv - Plant Biology 2020Quote: ... and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
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bioRxiv - Biophysics 2021Quote: ... The protein purification was carried out using a cation exchange HiTrap SP column (GE Healthcare, Chicago, IL) with phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded on a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... These fractions were then further purified using a Superdex 75 26/60 column (GE healthcare, IL, USA), and the fractions containing the recombinant protein were combined ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Immunology 2021Quote: ... and concentrated and further purified on a standardized Superdex 200 16/600 (GE Healthcare, Chicago, IL, USA) column running in HBS-E (10 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... the solution was loaded onto a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ABCA4 antibody (CL2) to CNBr-activated Sepharose 4B beads (GE Healthcare Bio-Sciences, Chicago, IL, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescence visualization was done with the Typhoon™ FLA 9000 laser scanner (GE Healthcare, Chicago, IL, USA). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Microbiology 2020Quote: ... the mixture containing DksA was pushed through a HiTrap SP column (GE Healthcare, Chicago, IL, United States) to bind the DksA protein and subsequently eluted with 50 mM – 1M gradient of sodium chloride ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was visualized using the ECL Select Western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm ...
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bioRxiv - Microbiology 2021Quote: ... and regeneration was performed in 10mM Glycine HCl (pH 1.5, GE Healthcare, Chicago, IL, BR-1003-54). Binding-affinity constants (KD ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged by passing the eluate through a desalting PD-10 column (GE Healthcare, Chicago, IL), equilibrated with 50 mM HEPES/KOH ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed on Superdex™ 200 Increase 5/150 GL (GE Healthcare, Chicago, IL).
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bioRxiv - Neuroscience 2019Quote: ... the membrane was developed using an ECL Prime Western Blotting Detection Reagent (GE Healthcare; Chicago, IL, USA). The density of protein bands from three independent experiments was quantified using the Image J software (National Institutes of Health [NIH] ...
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bioRxiv - Synthetic Biology 2020Quote: ... The OD600nm was determined in technical triplicates using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... a glutathione-S-transferase (GST) (64) and thrombin sequence derived from pGEX-2T (GE Healthcare, Chicago, IL), and a maltose binding protein (MBP ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was subjected to size exclusion chromatography using HiLoad 16/60 Superdex 200 (GE Healthcare, IL) with buffer containing 100 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... blots were developed with chemiluminescence substrate kit (Pierce ECL Western Blotting Substrate-GE Healthcare, Chicago, IL, USA) and the signal was captured by a CCD camera (ImageQuant LAS 4000 mini® ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed with TBST and incubated with enhanced chemiluminescence (ECL; GE Healthcare; Chicago, IL, USA) and signals were detected with on film (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fast protein liquid chromatography on the ÄKTA pure system (GE Healthcare Life Sciences, Chicago, IL) using protein A as the first chromatography step and ceramic hydroxyapatite (CHT ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged for 72h in an IN CELL Analyzer 2000 microscope (GE Healthcare, Chicago, IL, USA) equipped with temperature and CO2 controller ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The eluent was loaded into HiLoad 16/600 Superdex75™ pg column (GE Healthcare, Chicago, IL, USA) that was pre-equilibrated with buffer C [20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred to Amersham Protran premium 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA). Membranes were blocked with 5% skimmed milk (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... and quantified with a Typhoon 9400 scanner by ImageQuant Software version TL 8.1 (GE Healthcare, Chicago, IL). 3.6mg of protein per sample was reduced with 10mM Tris(2-carboxyethyl ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and cell pellets were re-suspended in 37% isotonic Percoll (GE Healthcare, IL, USA) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Proteins were analyzed on a Superdex 200 10/300 GL column from GE healthcare (Chicago, IL, USA) equilibrated using a mobile phase containing 150 mM KCl and 50 mM Tris-HCl pH 7.6 run at 0.4 ml/min ...
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bioRxiv - Pathology 2023Quote: ... immunoreactive bands were visualized using the enhanced chemiluminescence (ECL) detection reagent (GE Healthcare, Chicago, IL, USA, RPN2106) or acquired in the ImageQuant LAS 4000 device using the Immobilon Forte Western HRP substrate (WBLUF0100 ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was subsequently purified using a Superdex75 gel-filtration column (GE Healthcare, Chicago, IL, USA). Finally ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
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bioRxiv - Microbiology 2020Quote: ... Gels were washed in distilled water and imaged using an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA).
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bioRxiv - Developmental Biology 2020Quote: ... The membrane was washed again prior to exposure to ECL Western blot detection reagent (GE Healthcare, Chicago, IL) and GeneMate Blue Autoradiography Film (VWR ...