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Citations for GE Life Sciences :
701 - 750 of 1791 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with primary antibodies followed by peroxidase-conjugated secondary antibodies (GE HealthCare, Chicago, IL, USA). The antibodies utilized were rabbit monoclonal anti-pMEK2 (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were loaded onto a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare, Chicago, IL) equilibrated with 50 mM TRIS·HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... The mRNAs were further purified by filtration using MicroSpin™ S-200 HR columns (GE Healthcare, Chicago, IL, USA) to remove unreacted substrates (RNA reaction intermediates ...
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bioRxiv - Biochemistry 2021Quote: ... and radioactivity in bound and free RNA was quantified using Phosphorimager (GE) and ImageQuant 5.2 software (GE Healthcare, IL). Plots of the fraction bound RNA vs ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescent detection was completed with enhanced chemiluminescent (ECL) western blotting reagents (Catalog No. RPN2236, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2020Quote: ... LUVs were subjected to size exclusion chromatography on a sephadex G25 PD-10 column (GE Life Sciences, Chicago, Il). The concentration of the encapsulated Indo-1 was estimated using fluorescence and known amounts of free Indo-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Body fat percentage (BF%) was measured via dual-energy X-ray absorptiometry scans (LUNAR Prodigy; GE Healthcare, Chicago, IL) after overnight fasting.
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bioRxiv - Plant Biology 2022Quote: ... The resulting luciferase signal was detected with the digital camera system ‘ImageQuant LAS 4000’ (GE Healthcare, Chicago, IL, USA) and visualized with the open access software suite ‘Image J’ (National Institutes of Health ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were subjected to size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare, IL). The peak fractions were directly used for grid preparation with the reconstituted samples at ∼0.5 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: MRI scans were performed on each participant using a GE Discovery MR750 3T MRI scanner (GE Healthcare, Chicago, IL). No participant was in the scanner for longer than 120 minutes per session ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Cancer Biology 2019Quote: ... detection was achieved with ECL™ or ECL™ prime western blotting detection reagents (GE Healthcare, Chicago, IL, USA). The antibodies used were ...
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bioRxiv - Bioengineering 2019Quote: ... A 7 mm hole punch was used to cut pieces of blood separation membrane (MF1, GE Healthcare, Chicago, IL) and amplification membrane (0.22 µm PES ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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Structural integrity following focused ultrasound thalamotomy and its correlation with tremor reliefbioRxiv - Neuroscience 2019Quote: All MR imaging studies were performed using the same 3T system (Discovery™ MR750, GE Healthcare, Chicago, IL, USA). Patients were recruited between 2016– 2018 and scanned prior to the procedure and in four follow-up scans ...
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bioRxiv - Bioengineering 2020Quote: ... and the protein:antibody complexes were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2019Quote: ... Whatman® Nuclepore policarbonate filters with pore size of 100 nm were purchased from GE Healthcare (Chicago, IL, USA). Black 96-well plates with transparent bottom (lumox® ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... AAV particles was extracted in PBS and purified with an AKTA prime plus chromatography system (GE Healthcare, Chicago, IL) on an AVB Separose HP column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... sdRNA mimics and scrambled controls were ordered as custom miRIDIAN mimics from Dharmacon (GE Healthcare Dharmacon, Inc, Chicago, IL). Mimic and inhibitor sequences are detailed in Supplementary File 5 ...
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bioRxiv - Cell Biology 2021Quote: ... and the signal was captured by a CCD camera (ImageQuant LAS 4000 mini®, GE Healthcare, Chicago, IL, USA). As controls ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Genomics 2022Quote: ... 500 pmol of DNA oligonucleotides were immobilized using 20 μl of Streptavidin-Sepharose bead slurry (GE Healthcare, Chicago, IL). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... washed and dried prior to exposure and signal detection in a Typhoon FLA 9500 (GE Healthcare, Chicago, IL, USA) phosphoimager ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Chicago, IL, USA). Doxycycline ...
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bioRxiv - Molecular Biology 2023Quote: ... mice underwent echocardiography with a Vivid iQ R3 Premium and a L8-18i-RS transducer (GE Healthcare, Chicago, IL) under ketamine/xylazine anesthesia ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Developmental Biology 2022Quote: ... Band quantification was performed using the GE FujiFilm ImageQuant LAS-4000 CH mini Imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2023Quote: ... Detection was carried out using peroxidase-conjugated secondary antibodies and the ECL Plus Reagent (GE Healthcare, Chicago, IL, USA) or SuperSignal West Femto (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). T cells were negatively purified with EasySepTM beads (human T-cell isolation kit ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
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bioRxiv - Microbiology 2024Quote: ... MV samples were administered at six concentrations (1:3 dilutions in Neurobasal A (Hyclone, GE Healthcare, Chicago, IL, USA) containing Glutamax (Hyclone ...
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bioRxiv - Biophysics 2021Quote: ... centrifuged and loaded into a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with 40 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... were added to isolate the epithelial cells using MagRack 6 (GE Healthcare 89129-096), and the rest cells in the suspension were mesenchymal cells ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded onto a Superose 6 10/300 size exclusion chromatography column (GE Healthcare) pre-equilibrated with a preparation buffer containing 25 mM K·HEPES ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated after 6% dextran sedimentation and Ficoll-Paque Plus (GE Healthcare®) density gradient separation ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2019Quote: ... Nanodisc reconstituted TRPV2 was further purified using size-exclusion chromatography (Superose 6, GE Healthcare) in Wash Buffer ...