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Citations for GE Life Sciences :
251 - 300 of 1336 citations for IL 3RA Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1mL His-Trap FF column (GE Healthcare Life Sciences) and ZapB was eluted with an imidazole gradient (0 to 0.5M) ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated samples were loaded onto Hi Load 16/600 Superdex 75 pg column (GE Healthcare) connected to the NGC Scout Chromatography system and running buffer composed of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was applied to a Hi-Load Superdex 200 16/60 column (GE Healthcare) in a buffer A ...
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bioRxiv - Microbiology 2020Quote: ... And further purified by gel filtration on a Hi Load Superdex 75 column (GE Healthcare). Peak fractions were concentrated to 10 mg/ml in 20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... RBD protein was then captured using His-Trap HP column (GE Healthcare, Wauwatosa, WI, USA) or HiTrap TALON crude column (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant (crude extract) loaded on a 1-ml His-Trap column (GE Healthcare) previously equilibrated with Buffer H ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was then loaded onto a His-Trap column (GE Healthcare Life Sciences) and eluted with buffer B (100nM NaCl and 500 mM imidazole in 50 mM Tris at pH 8.0) ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was then applied to a nickel-affinity chromatography (His Trap excel, GE Healthcare) equilibrated with buffer B (20mM Tris pH 7.2 ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... The His-tagged proteins were purified by cobalt affinity chromatography using Chelating Sepharose (GE Healthcare). The proteins were eluted in buffer containing 25 mM Hepes-KOH (pH 7.4) ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Biochemistry 2022Quote: ... and the filtrate was loaded on to a Hi Trap SP HP column (GE healthcare) equilibrated in buffer B1 [50 mM Na2HPO4 at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on an equilibrated 5 mL His-trap column (GE healthcare/Cytiva) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins in the supernatants were purified using HIStrap FF columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... Thawed cells were spun and Ku was bound to His Mag Sepharose Ni (GE Healthcare). Beads were washed in Lysis buffer containing 50 mM imidazole and Ku was eluted in Lysis buffer with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then passed through a 5 mL His-Trap FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... the samples were loaded on a Hi-Load Superdex 200 16/600 column (GE Healthcare) equilibrated with 50mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... Image was acquired with Image Quant LAS 4000 (GE Healthcare, IL). The band intensity was quantified with ImageJ (NIH).
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bioRxiv - Plant Biology 2019Quote: ... were precipitated using the 2D Cleanup kit (GE Healthcare, Chicago, IL), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... amplicons were purified using illustra ExoProStar (GE Healthcare, Chicago IL, USA) and sequenced using the BigDye Terminator Cycle Sequencing Kit v 3.1 (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using ECL Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... and anion exchange column HiTrap Q HP (GE Healthcare, IL, USA). All tags for purification were cut and removed ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Membrane was sequentially probed overnight with primary antibodies (see Supplementary Table 6 ...
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bioRxiv - Neuroscience 2019Quote: ... 10% fetal bovine serum (FBS; GE Healthcare Life Sciences, Chicago, IL), 5% horse serum (HS ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 5% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL), 2 mM L-glutamine (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Following blocking ...
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bioRxiv - Cell Biology 2021Quote: ... followed by chemiluminescent ECL detection (Amersham, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Plant Biology 2022Quote: ... and a desalting column (PD-10, GE Healthcare, Chicago, IL, USA) was used for removing sucrose and for buffer exchange ...
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bioRxiv - Microbiology 2022Quote: ... following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare, Chicago, IL) for 25 minutes at 500 × g.
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated using Ficoll-paque plus (GE Healthcare, Chicago, IL). CD8+ T cells were purified using CD8+ Isolation Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5% fetal bovine serum (HyClone, GE Healthcare, Chicago, IL) and 10 μg/ml gentamicin (MilliporeSigma ...
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bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated anti-mouse-IgG antibody (GE Healthcare, Chicago, IL, USA), diluted 1:5,000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... purified using an S-400 spin column (GE Healthcare, IL, USA), ligated ...
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bioRxiv - Microbiology 2021Quote: ... using a HiTrap Chelating HP column (GE Healthcare, Chicago, IL, USA) loaded with Zn2+ ions and equilibrated with low-histidine buffer ...
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bioRxiv - Cell Biology 2019Quote: ... an IN Cell Analyzer 2000 automated imager (GE Healthcare, Chicago, IL), was used for sample imaging.