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Citations for GE Life Sciences :
151 - 200 of 2029 citations for IL 3 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
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bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then run through an equilibrated His-trap (GE healthcare) in an AKTA start fast protein liquid chromatography system ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a 5mL His-trap column (GE Healthcare) for protein purification and eluted with a linear imidazole (20 mM-200 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was purified by His TrapTM FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... visualized with Supersignal West Pico Chemiluminscent Substrate (Pierce/ThermoScientific; IL, USA) by using the enhanced chemiluminescence imager: LAS400 (GE Healthcare, IL, USA). Membranes were then stripped and reprobed for quantitation of the corresponding total protein or GAPDH to allow for normalization of values based on total protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... and detected with ECL Prime (GE Healthcare, Chicago, IL) and a CCD camera (G:BOX Chemi XT4 ...
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bioRxiv - Biochemistry 2020Quote: ... 1ml Sephadex G-50 (GE Healthcare Life Sciences, IL), which was equilibrated with 50 mM Tris-HCl (pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins transferred onto nitrocellulose (GE Healthcare, Chicago, IL) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2021Quote: ... transferred to hybond PVDF membrane (GE Healthcare, Chicago, IL), and incubated with the following antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... using a HiTrap Desalting column (GE Healthcare, Chicago, IL). Labeled lipoproteins were collected in the void volume.
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bioRxiv - Biophysics 2019Quote: ... pore size 0.45 μm (GE Healthcare, Chicago IL, USA). Membrane was blocked with 5% fat free milk in TBS and incubate overnight at 4°C with Vimentin antibody (dilution 1:1000 in BSA 5% ...
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bioRxiv - Synthetic Biology 2020Quote: 50 nmol of Cy5-maleimide (GE Healthcare, Chicago, IL) dissolved in anhydrous DMSO (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... followed by ECL prime (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2020Quote: ... Ficoll was purchased from GE healthcare (Chicago, IL, USA), ultrapure water ...
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bioRxiv - Biophysics 2022Quote: ... a PD-10 desalting column (GE Healthcare, Chicago, IL) was used to remove excess imidazole and buffer exchange in between the Ni affinity purification and thrombin cleavage ...
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bioRxiv - Pathology 2023Quote: ... purified by 30% Percoll (GE Healthcare, Chicago, IL; 17089101) density gradient separation ...
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bioRxiv - Microbiology 2023Quote: ... 90V onto nitrocellulose membranes (GE Healthcare, Chicago, IL USA). The blocked membranes were probed with either rabbit polyclonal MMTV anti-Gag antibody (Rockland Immunochemicals ...
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bioRxiv - Biochemistry 2023Quote: ... Superdex 200 pg column (GE Healthcare, Chicago, IL, USA) using acylase buffer without glycerol.
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bioRxiv - Cell Biology 2023Quote: ... Glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL, USA); and reduced glutathione (Fujifilm) ...
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bioRxiv - Cell Biology 2023Quote: ... using an Amersham Imager 600 (GE Healthcare, Chicago, IL) imaging system ...
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bioRxiv - Biochemistry 2023Quote: ... Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) was then used to capture the 6×His-GB1 and 6×His-TEV ...
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bioRxiv - Biochemistry 2023Quote: ... on an Äkta explorer FPLC (GE Healthcare, Chicago, IL). The column was washed with 12 column volumes (CV ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was collected for purification using His Trap Excel column (GE Healthcare). The proteins were labeled with an AlexaFluor dye through the ybbR-tag and gel-filtered via a Superdex 75 10/300 GL column ...
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bioRxiv - Cell Biology 2019Quote: ... His-NF1 GAP was purified using a HisTrap excel-affinity column (GE Healthcare) and eluted in buffer containing 50mM Tris ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Neuroscience 2020Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on a Ni-NTA His-trap column (GE healthcare), washed with 10 column volume of buffer A containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Biochemistry 2021Quote: IMAC purification was performed using a His-Trap HP 1 ml (GE Healthcare) on an ÄKTA START protein purification system (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and applied to a 1-ml chelating Hi-Trap Sepharose column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...