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Citations for GE Life Sciences :
201 - 250 of 1711 citations for IL 2 Rat HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Bands were detected using enhanced chemiluminescence (Pierce Biotechnology, IL, USA) and imaged with a LAS 400 Mini Imaging System (GE Healthcare, IL, USA). Immuno-positive bands were quantified using ImageJ software (NIH ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 0.2% BSA in TBST) incubation followed by chemiluminescence-based detection (Pierce, Rockford, IL) with an Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL) (24).
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Hi-Load 16/600 Superdex 200 prep grade column (GE Healthcare Bioscience) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... tNPR1-His proteins were further purified by HiLoad 16/600 Superdex 200 (GE Healthcare) with 200 mM NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... and supernatants loaded onto 5 mL HP HIS-TRAP columns (GE Healthcare Life Sciences) equilibrated in buffer A (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was loaded onto a 5mL His Trap HP column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4 and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of immobilised E141A H164A OmCI protein ...
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bioRxiv - Cell Biology 2020Quote: ... the elution was further purified by Hi-trap Q anion-exchange chromatography (GE Healthcare). Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... the elution was further purified by Hi-trap Q anion-exchange chromatography (GE Healthcare). Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The crude extract was applied onto a nickel chelation column (His GraviTrap, GE Healthcare), and TasA28-261-His was eluted according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Synthetic Biology 2021Quote: ... then imaged using a DeltaVision microscope (GE Healthcare, Chicago, IL) equipped with a 100x objective (Olympus ...
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bioRxiv - Evolutionary Biology 2021Quote: ... quantified with a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA), and stored at −80°C ...
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bioRxiv - Systems Biology 2020Quote: ... blots were flooded with ECL (Amersham; GE Healthcare, Chicago, IL) and bands detected using chemiluminescent film (Hyperfilm ECL ...
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bioRxiv - Microbiology 2019Quote: ... Superose 6 10/300 GL (GE Healthcare, Chicago, IL, USA) equilibrated with 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and a Superdex 200 size exclusion chromatography (GE Healthcare, IL). The peak fractions were concentrated to 0.1 mg/mL for DXT (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... and Superdex 75 gel filtration chromatography (GE Healthcare, Chicago, IL) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... M13KO7 helper phage were obtained from GE Healthcare (Chicago, IL). Ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... gels were soaked in Amplify solution (GE Healthcare, Chicago, IL) for 30 min before drying on a vacuum air dryer ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 ml Sephadex G-50 (GE Healthcare Life Sciences, IL) in order to remove excess NHS-fluorescein.
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bioRxiv - Neuroscience 2019Quote: ... 5% horse serum (HS; GE Healthcare Life Sciences, Chicago, IL), 1% Pen-Strep (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with Glutathione Sepharose (GE Healthcare, Chicago, Il) for 90 minutes after which beads were washed with IPB ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Penicillin/Streptomycin (#sv30010, HyClone, GE Healthcare, Chicago, IL). The mantle cell lymphoma cell line Maver-1 (#ACC 717 ...
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bioRxiv - Neuroscience 2022Quote: A 3.0T MRI scanner (GE 750; GE Healthcare, Chicago, IL) with equal imaging protocol settings at all time points was applied to acquire axial head 3-dimensional T1-weighted ...
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bioRxiv - Molecular Biology 2019Quote: ... and imager ImageQuant LAS 4000 (GE Healthcare; Chicago, IL, USA). Primary and secondary antibodies used in this study are summarized in Table 3.
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bioRxiv - Plant Biology 2020Quote: ... Horseradish peroxidase –conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were visualized using ECL reagent (GE Healthcare, Chicago, IL) and immunoblot intensities were quantified with ImageJ.[32].