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Citations for GE Life Sciences :
451 - 500 of 1331 citations for IL 2 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... we performed Western blotting per the protocol provided with the ECL (Enhanced Chemiluminescence) Detection System (GE Healthcare, IL). Furthermore ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Analyses were performed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) (GE Healthcare Life Sciences, Chicago, IL), and then the membranes were scanned using Image Quant LAS4010 (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were isolated from whole blood by density-gradient centrifugation using Ficoll-Paque™ (GE Healthcare, Chicago, IL). Total cellular DNA was extracted and used in subsequent PCR experiments.
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The specific band was detected using the ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA) with the ImageQuant LAS 4000 digital imaging system (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were purified from the remaining cells by overlaying on a Ficoll density gradient (GE Healthcare, Chicago, IL) following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... The homogenate was clarified by centrifugation and mixed with Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL). After extensive washing ...
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bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
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bioRxiv - Immunology 2022Quote: ... the complex was purified by size exclusion chromatography (Superdex 200 Increase size exclusion column, GE Healthcare, Chicago, IL) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... and 0.1 mM EDTA] was further purified by using Hitrap Q HP column (GE Healthcare, Chicago, IL, USA) and AKTA start (GE Healthcare) ...
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bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
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bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
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bioRxiv - Molecular Biology 2020Quote: ... The NPC suspension was subjected to a density gradient centrifugation using Percoll (Lot 10221921, GE Healthcare, Chicago, IL) at 900 xg for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Molecular Biology 2019Quote: Imaging was performed on the high-resolution GE Discovery 690 ADC PET/CT System (GE Healthcare, Chicago, IL) at 6 ...
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bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare, Chicago, IL, USA) using 25 mM NaPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... the elution was desalted into 50 mM ABC buffer using HiPrep Desalting column (GE Healthcare: Chicago, IL, USA) and lyophilized into 100 nmol per aliquot.
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... Hybridising bands were visualized using the ECL™ Select western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm España ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was developed using the Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Chicago, IL USA), and the signal was detected using an OPTIMAX film processor (Protec ...
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bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
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bioRxiv - Biochemistry 2022Quote: Clarified supernatants were applied to an equilibrated HiPrep Q Fast Flow 16/10 column (GE Healthcare, Chicago, IL). Eluted proteins were collected ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
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bioRxiv - Biochemistry 2023Quote: ITC experiments were carried out on dA using a high-precision ITC200 microcalorimeter (GE Healthcare, Chicago, IL, USA). Arginine binding was measured at 25 °C in HEPES 10 mM ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Cell Biology 2023Quote: ... The VacA-containing soluble fraction was loaded onto an anion exchange column (DEAE Sephacel, GE healthcare, Chicago, IL) pre-equilibrated with wash buffer ...
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bioRxiv - Neuroscience 2023Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Body composition was measured with dual-energy X-ray absorptiometry (DXA, LUNAR Prodigy, GE Healthcare, Chicago, IL, USA). Measures of total (LBM) ...
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bioRxiv - Biochemistry 2023Quote: Obtained gels and membranes were analyzed in Image Master 2D Platinum 7 Software (GE Healthcare, Chicago, IL, USA) to look for qualitative and/or quantitative differences in abundance/antigenicity of some venom proteins between the samples ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with primary antibodies followed by peroxidase-conjugated secondary antibodies (GE HealthCare, Chicago, IL, USA). The antibodies utilized were rabbit monoclonal anti-pMEK2 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were loaded onto a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare, Chicago, IL) equilibrated with 50 mM TRIS·HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... The mRNAs were further purified by filtration using MicroSpin™ S-200 HR columns (GE Healthcare, Chicago, IL, USA) to remove unreacted substrates (RNA reaction intermediates ...
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bioRxiv - Biochemistry 2021Quote: ... and radioactivity in bound and free RNA was quantified using Phosphorimager (GE) and ImageQuant 5.2 software (GE Healthcare, IL). Plots of the fraction bound RNA vs ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...