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Citations for GE Life Sciences :
351 - 400 of 1756 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Cell Biology 2019Quote: ... His-SEPT2-mCherry-SEPT6/7-strep was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... an initial IMAC step was performed on a His-Trap column (all columns by GE Healthcare) using buffers A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: Proteins were purified according to their His6-tag using a His Excel column from GE Healthcare attached to a Äkta Start system ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Cell Biology 2022Quote: ... the elution fractions containing His-ZNF524 were further purified on a HiTrap Heparin HPTM (GE Healthcare). Therefore ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Cell Biology 2020Quote: ... and ImageQuant LAS4000 Mini software (GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... Blots were developed using ECL Prime (RPN 2232, GE Healthcare, Il, USA). The chemiluminescence signals were detected by Las-4,000 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... and transferred onto PVDF membranes (GE Healthcare, Cat#15269894, Chicago, IL, USA) using a semi-dry transfer blotter (Bio-rad) ...
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bioRxiv - Immunology 2019Quote: ... Mononuclear cells were isolated on Ficoll-Paque PLUS (GE Healthcare, Chicago, IL) and stimulated in complete RPMI using 1% phytohemagglutinin (Invitrogen) ...
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bioRxiv - Immunology 2019Quote: ... These were further subjected to Ficoll (GE Healthcare Life Sciences, Chicago, IL) density gradient centrifugation at 400 x g for 30 minutes to isolate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Neuroscience 2019Quote: ... Excess DTT was removed using NAP-5 column (GE Healthcare, Chicago, IL) with PBS (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... on a Superdex 200 Increase 10/300GL (GE Healthcare, Chicago, IL, USA) column in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and NHS-activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences, IL), was added to the sample ...
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bioRxiv - Biophysics 2022Quote: ... and loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... both mounted in an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). The sample was concentrated to 100 μM using an Amicon Ultra 15 mL centrifugal filter (Merck KGaA ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were enriched using Ficoll-Paque PLUS (GE Healthcare, Chicago IL, USA) density gradient centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... and detected using an Amersham Imager 600 (GE Healthcare, Chicago, IL, USA). After band detection ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were detected using Amersham ECL reagent (GE Healthcare, Chicago, IL, USA) and ChemiDoc imager (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Amersham ECL prime immunoblotting detection reagents (#RPN2236, GE Healthcare, Chicago, IL, USA) were used to detect immunoreactive bands on a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE Healthcare, IL, USA). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were purified by Ficoll® Paque Plus (GE Healthcare, Chicago, IL) gradient centrifugation for 30 min at 2800 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was added to glutathione Sepharose 4B (GE Healthcare, Chicago, IL) and incubated 4 h at 4°C under agitation ...
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bioRxiv - Biochemistry 2020Quote: ... was covalently immobilized on a CM5 sensor chip (GE Healthcare, Chicago, IL) by amine coupling ...
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bioRxiv - Physiology 2020Quote: ... was used in an ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA) for imaging of blots by chemiluminescence ...
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bioRxiv - Neuroscience 2020Quote: ... and quantified using the Amersham Imager 600 (GE Healthcare, Chicago, IL, USA). The optical density of GR was normalised to β-actin and a cross-membrane control.
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bioRxiv - Pathology 2020Quote: ... which were then transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA). After blocking with 8% non-fat dry milk in TBST for 2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... An anti-Rabbit-IgG HRP linked whole antibody (GE Healthcare, Chicago, IL) was used as a secondary antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on a S200 sizing column (GE Healthcare, Chicago, IL) in HNT buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Neuroscience 2021Quote: ... The nitrocellulose membrane and Whatman filters (all GE Healthcare, Chicago, Il, USA) were washed 2X with Milli-Q H20 and 2X with Tris-Buffered Saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... they were soaked in PlusOne Repel-Silane ES (GE Healthcare; Chicago IL) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS; HyClone, GE Healthcare, Chicago, IL), 40 μg/ml gentamicin and 0.01M Tricin (both from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were overlaid onto Ficoll-Paque Plus (GE Healthcare, Chicago, IL) at a ratio of 2.8:1 and centrifuged at 400 × g for 30 minutes to remove red blood cell contamination ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 mL HiTrap™ chelating HP column (GE Healthcare, Chicago, IL, USA) charged with Ni2+ was used ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 5 mL of 40% Percoll (GE Healthcare, Chicago, IL, USA), and underlaid with 5 mL of 80% Percoll (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... followed by wet transfer to a nitrocellulose membrane (GE Healthcare, Chicago, IL). Total protein was stained and destained by Pierce Reversible Protein Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Imaging was performed using ECL prime reagent (GE healthcare, Chicago, IL, USA) with an Azure c600 instrument (Azure Biosystems ...
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bioRxiv - Pathology 2023Quote: ... Proteins were blotted onto nitrocellulose membranes (Amersham, GE Healthcare, Chicago, IL, USA). Western blotting was performed with the use of primary antibodies listed in Table 2 ...
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bioRxiv - Biochemistry 2023Quote: ... The product was purified using PD-10 columns (GE Healthcare, Chicago, IL) in deionized water containing 3 mM BME ...
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bioRxiv - Cell Biology 2022Quote: ... PBMCs were obtained by Ficoll-Paque PLUS (GE Healthcare, Chicago, IL, USA)-assisted density gradient centrifugation ...