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Citations for GE Life Sciences :
101 - 150 of 943 citations for IL 17RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 5% horse serum (HS; GE Healthcare Life Sciences, Chicago, IL), 1% Pen-Strep (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with Glutathione Sepharose (GE Healthcare, Chicago, Il) for 90 minutes after which beads were washed with IPB ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Penicillin/Streptomycin (#sv30010, HyClone, GE Healthcare, Chicago, IL). The mantle cell lymphoma cell line Maver-1 (#ACC 717 ...
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bioRxiv - Neuroscience 2022Quote: A 3.0T MRI scanner (GE 750; GE Healthcare, Chicago, IL) with equal imaging protocol settings at all time points was applied to acquire axial head 3-dimensional T1-weighted ...
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bioRxiv - Molecular Biology 2019Quote: ... and imager ImageQuant LAS 4000 (GE Healthcare; Chicago, IL, USA). Primary and secondary antibodies used in this study are summarized in Table 3.
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bioRxiv - Plant Biology 2020Quote: ... Horseradish peroxidase –conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were visualized using ECL reagent (GE Healthcare, Chicago, IL) and immunoblot intensities were quantified with ImageJ.[32].
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bioRxiv - Plant Biology 2019Quote: ... filled with Sephadex G-50 superfine (GE Healthcare, Chicago, IL) was run on a Bio-Rad BioLogic Duo Flow FPLC system equipped with a QuadTec detector ...
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bioRxiv - Cancer Biology 2020Quote: ... and immobilized onto nitrocellulose membranes (GE Healthcare, Chicago, IL, USA) by electroblot (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... visualized using ImageQuant LAS 500 (GE Healthcare, Chicago, IL, US) then quantified relatively to loading controls by optical density using ImageJ (V1.46d ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were soaked in Amplify (GE Healthcare, Chicago, IL, USA), dried and exposed to films (Carestream Health Medical X-ray Blue ...
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bioRxiv - Physiology 2020Quote: ... or ECL™ Prime reagent kits (GE Healthcare, Chicago, IL). Western blots were first probed with the antibody of interest ...
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bioRxiv - Microbiology 2019Quote: ... and exposed to a phosphor screen (GE Healthcare, Chicago, IL) overnight ...
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bioRxiv - Plant Biology 2020Quote: ... and purified using Glutathione Sepharose 4B resin (GE healthcare, IL). Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Microbiology 2020Quote: ... or anti-rabbit Ig-HRP (GE Healthcare, Chicago, IL, USA) both diluted to 1:5,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated in streptavidin-Cy5 (GE Healthcare, Chicago, IL, USA) [16] ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated with Ficoll-Hypaque (GE Healthcare, Chicago, IL) density gradient centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... 10% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL, USA), and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (P/S ...
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bioRxiv - Plant Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were obtained from GE Healthcare (Chicago, IL, USA). Bound antibodies were detected by ECL (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Plant Biology 2020Quote: ... Image was acquired with Image Quant LAS 4000 (GE Healthcare, IL). The band intensity was quantified with ImageJ (NIH).
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bioRxiv - Plant Biology 2019Quote: ... were precipitated using the 2D Cleanup kit (GE Healthcare, Chicago, IL), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... amplicons were purified using illustra ExoProStar (GE Healthcare, Chicago IL, USA) and sequenced using the BigDye Terminator Cycle Sequencing Kit v 3.1 (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using ECL Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... and anion exchange column HiTrap Q HP (GE Healthcare, IL, USA). All tags for purification were cut and removed ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Membrane was sequentially probed overnight with primary antibodies (see Supplementary Table 6 ...
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bioRxiv - Neuroscience 2019Quote: ... 10% fetal bovine serum (FBS; GE Healthcare Life Sciences, Chicago, IL), 5% horse serum (HS ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 5% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL), 2 mM L-glutamine (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Following blocking ...
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bioRxiv - Cell Biology 2021Quote: ... followed by chemiluminescent ECL detection (Amersham, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Plant Biology 2022Quote: ... and a desalting column (PD-10, GE Healthcare, Chicago, IL, USA) was used for removing sucrose and for buffer exchange ...
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bioRxiv - Microbiology 2022Quote: ... following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare, Chicago, IL) for 25 minutes at 500 × g.
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated using Ficoll-paque plus (GE Healthcare, Chicago, IL). CD8+ T cells were purified using CD8+ Isolation Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5% fetal bovine serum (HyClone, GE Healthcare, Chicago, IL) and 10 μg/ml gentamicin (MilliporeSigma ...
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bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated anti-mouse-IgG antibody (GE Healthcare, Chicago, IL, USA), diluted 1:5,000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... purified using an S-400 spin column (GE Healthcare, IL, USA), ligated ...
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bioRxiv - Microbiology 2021Quote: ... using a HiTrap Chelating HP column (GE Healthcare, Chicago, IL, USA) loaded with Zn2+ ions and equilibrated with low-histidine buffer ...
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bioRxiv - Cell Biology 2019Quote: ... an IN Cell Analyzer 2000 automated imager (GE Healthcare, Chicago, IL), was used for sample imaging.
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bioRxiv - Pathology 2020Quote: ... The Encore v.13 2011 (GE Healthcare Systems, Chicago, IL, USA) software system was used for data analysis ...
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bioRxiv - Neuroscience 2019Quote: ... Images were acquired automatically using an InCell2200 (GE Healthcare, Chicago, IL) or a Cell Insight CX5 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... ECL Prime Western Blotting Detection Reagent (GE Healthcare; Chicago, IL, USA) was used to develop the membrane ...
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bioRxiv - Cell Biology 2019Quote: ... HisTRAP FF and DEAE columns were from GE Healthcare (Chicago, IL).