Labshake search
Citations for GE Life Sciences :
501 - 550 of 943 citations for IL 17RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
-
bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
-
bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
-
bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
-
bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
-
bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
-
bioRxiv - Microbiology 2020Quote: ... Gels were washed in distilled water and imaged using an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA).
-
bioRxiv - Developmental Biology 2020Quote: ... The membrane was washed again prior to exposure to ECL Western blot detection reagent (GE Healthcare, Chicago, IL) and GeneMate Blue Autoradiography Film (VWR ...
-
bioRxiv - Genomics 2020Quote: ... PBMCs were isolated from blood samples by Ficoll-Paque™ PLUS density gradient centrifugation (GE Healthcare, Chicago, IL) and frozen in fetal bovine serum (Gibco ...
-
bioRxiv - Immunology 2019Quote: ... The eluted protein was concentrated and loaded on a Superdex 200 16/60 column (GE Healthcare, Chicago, IL) pre-equilibrated with PBS ...
-
bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
-
bioRxiv - Molecular Biology 2019Quote: ... we performed Western blotting per the protocol provided with the ECL (Enhanced Chemiluminescence) Detection System (GE Healthcare, IL). Furthermore ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
-
bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
-
bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Analyses were performed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) (GE Healthcare Life Sciences, Chicago, IL), and then the membranes were scanned using Image Quant LAS4010 (GE Healthcare Life Sciences) ...
-
bioRxiv - Microbiology 2020Quote: ... PBMCs were isolated from whole blood by density-gradient centrifugation using Ficoll-Paque™ (GE Healthcare, Chicago, IL). Total cellular DNA was extracted and used in subsequent PCR experiments.
-
bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... The specific band was detected using the ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA) with the ImageQuant LAS 4000 digital imaging system (GE Healthcare).
-
bioRxiv - Microbiology 2022Quote: ... Neutrophils were purified from the remaining cells by overlaying on a Ficoll density gradient (GE Healthcare, Chicago, IL) following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare ...
-
Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... The homogenate was clarified by centrifugation and mixed with Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL). After extensive washing ...
-
bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
-
bioRxiv - Immunology 2022Quote: ... the complex was purified by size exclusion chromatography (Superdex 200 Increase size exclusion column, GE Healthcare, Chicago, IL) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2019Quote: ... and 0.1 mM EDTA] was further purified by using Hitrap Q HP column (GE Healthcare, Chicago, IL, USA) and AKTA start (GE Healthcare) ...
-
bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
-
bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
-
bioRxiv - Molecular Biology 2020Quote: ... The NPC suspension was subjected to a density gradient centrifugation using Percoll (Lot 10221921, GE Healthcare, Chicago, IL) at 900 xg for 20 minutes at room temperature ...
-
bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
-
bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
-
bioRxiv - Molecular Biology 2019Quote: Imaging was performed on the high-resolution GE Discovery 690 ADC PET/CT System (GE Healthcare, Chicago, IL) at 6 ...
-
bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare, Chicago, IL, USA) using 25 mM NaPO4 ...
-
bioRxiv - Biochemistry 2021Quote: ... the elution was desalted into 50 mM ABC buffer using HiPrep Desalting column (GE Healthcare: Chicago, IL, USA) and lyophilized into 100 nmol per aliquot.
-
bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
-
bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
-
bioRxiv - Microbiology 2022Quote: ... Hybridising bands were visualized using the ECL™ Select western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm España ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was developed using the Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Chicago, IL USA), and the signal was detected using an OPTIMAX film processor (Protec ...
-
bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
-
bioRxiv - Biochemistry 2022Quote: Clarified supernatants were applied to an equilibrated HiPrep Q Fast Flow 16/10 column (GE Healthcare, Chicago, IL). Eluted proteins were collected ...
-
bioRxiv - Cell Biology 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
-
bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
-
bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
-
bioRxiv - Biochemistry 2023Quote: ITC experiments were carried out on dA using a high-precision ITC200 microcalorimeter (GE Healthcare, Chicago, IL, USA). Arginine binding was measured at 25 °C in HEPES 10 mM ...
-
bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...