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Citations for GE Life Sciences :
501 - 550 of 1336 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant containing cDNA-linker-VHH complex was purified using His Mag Sepharose Ni beads (GE Healthcare, USA). The supernatant was added to 20 μL of the beads and incubated at 25°C for 30 min ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and the His-tagged sec49K was purified with HisTrap excel Ni beads (GE Healthcare), eluted with 300 mM imidazole and dialysed against buffer containing 50% glycerol (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag was separated from DBD on an S75 10/300 GE gel filtration column (GE healthcare) equilibrated with 10 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on an affinity chromatography column (GE Healthcare, His-Trap FF crude, #17-5286-01) with a flow rate of 1 mL/ min ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Immunology 2022Quote: ... and HEK293 cell lines were obtained from Royan Institute Cell Bank and cultured in Dulbecco’s Modified Eagle Medium (DMEM, GE Healthcare Life Sciences, Pittsburgh, PA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Biophysics 2019Quote: ... SEC experiments were carried out using a Superose 12 (GE Healthcare, Chicago, IL, US) column equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... Purification was performed by gel filtration chromatography (Superdex 75: GE Healthcare, Chicago, IL, US) in 20 mM HEPES ...
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bioRxiv - Developmental Biology 2020Quote: ... and proteins were detected using Amersham Western Blotting Detection Reagent (GE Healthcare, Chicago, IL). Western blot membranes were probed with mouse anti-RFP (RF5R Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Densitometric analysis of imaged blots was performed using Imagequant TL (GE Healthcare, IL, USA). For obtaining relative densitometry ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389 ...
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bioRxiv - Biochemistry 2020Quote: All ITC titrations were performed on a MicroCal iTC 200 instrument (GE Healthcare, IL). All reactions were performed in 20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... B16-F10 and MC38 cells were cultured in DMEM (HyClone, GE Healthcare, Chicago IL) with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2019Quote: Cell OD600 was measured using an Ultrospec 1100 pro spectrophotometer (GE Healthcare, Chicago IL). Yeast cell biomass was determined by cell dry weight measurement from 2 ml of culture samples ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... containing 10% vol/vol fetal bovine serum (#SH3062601, HyClone, GE Healthcare, Chicago, IL USA). The tube was then centrifuged at 1600 rpm for 10 minutes at 4°C and the pellet resuspended in Hank’s Buffered Saline Solution (#14175095 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were detected using the enhanced chemiluminescence (ECL) reagent (GE Healthcare, Chicago, IL, USA) or Western Lightning Plus-ECL reagents (Perkin Elmer ...
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bioRxiv - Biophysics 2021Quote: ... lysate was purified on a 1 ml Strep Trap HP (GE Healthcare, Chicago, IL) using the Äkta pure purification system (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by an anti-rabbit-IgG HRP linked whole antibody (GE Healthcare, Chicago, IL). The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The blots were developed using Amersham ECL detection reagent (GE Healthcare, Chicago, IL, USA). For samples derived from human islets ...
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bioRxiv - Cancer Biology 2020Quote: ... then imaged via an Image Quant LAS 4010 chemiluminescence imager (GE Healthcare, Chicago, IL). Band intensities were quantified using ImageJ and normalized to β-actin intensities.
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bioRxiv - Cell Biology 2022Quote: ... bone marrow was overlayed on Ficoll-paque density gradient medium (GE healthcare, Chicago, IL), washed twice with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... The eluted proteins were further purified via gel filtration (Superdex200, GE Healthcare Chicago, IL).
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bioRxiv - Immunology 2022Quote: ... media was filtered through 0.2 μm PVDF filtered syringes (GE Healthcare, Chicago, IL, USA) and centrifuged at 120,000 x g for 90 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... or on polyacrylamide gels with the PlusOne silver staining kit (GE Healthcare, Chicago, IL). Size exclusion chromatography was performed on Superdex™ 200 Increase 5/150 GL (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... Di-luted blood was then layered over Ficoll-Paque PLUS (GE Healthcare, Chicago, IL) for density centrifugation using Sepmate tubes (Stemcell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The mAbs were purified using HiTrap Protein A HP column (GE Healthcare, Chicago, IL), followed by size exclusion chromatography (Superdex 200 16/90 ...
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bioRxiv - Microbiology 2020Quote: ... the culture fluids were harvested and purified by protein G (GE Healthcare, Chicago, IL). The concentration of purified IgG was calculated by measuring the absorbance at 280 nm ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was then flowed over a Q sephaorse (GE Healthcare, Chicago, IL, US, 17051001) column ...
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bioRxiv - Neuroscience 2022Quote: ... followed by detection with a FujiFilm ImageQuant LAS-4000 detector (GE Healthcare, Chicago, IL).
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bioRxiv - Neuroscience 2022Quote: ... and imaged with an Amersham Imager 680 imaging system (GE Healthcare, Chicago, IL, USA). Bands were analyzed by densitometry ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were observed in a GE DeltaVision OMX-SR microscope (GE Healthcare, Chicago, IL). Images were analyzed using Fiji Software (National Institutes of Health ...
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bioRxiv - Microbiology 2022Quote: ... the gel was electroblotted onto a nylon transfer membrane (GE Healthcare, Chicago, IL, USA) at 300 mA for 60 min and crosslinked with a UV crosslinker at 120 mJ for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... The slides were exposed to phosphor plates (BAS 2040, GE Healthcare, Chicago, IL, USA) for 1 day and the ARG images were digitized by the Typhoon FLA 7000 (West Avenue ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA) by wet blotting ...
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei counting was performed using an INCell Analyzer 2200 (GE Healthcare, Chicago, IL, USA) cell imaging system ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were resuspended in 10 ml of 40% Percoll (GE Healthcare, Chicago, IL) and centrifuged again at 950 x g for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and transferred to a nitrocellulose membrane (Hybond™-P, GE Healthcare, Chicago, IL, USA) using the semi-dry Trans-Blot® Turbo™ Transfer System (Bio-Rad ...