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Citations for GE Life Sciences :
51 - 100 of 160 citations for IGF I Human G49 A118 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was isolated from samples using illustra RNAspin Mini RNA Isolation Kit with RNase-free DNase I (GE Healthcare) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... with 2% FBS and 0.5 mg/mL DNase I) and endothelial cells were obtained using density gradient centrifugation with 22% Percoll (GE Healthcare) in PBS (1X) ...
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bioRxiv - Zoology 2021Quote: Transthoracic echocardiographic examinations were performed in the orangutans’ indoor enclosure in the Ménagerie, le Zoo du Jardin des Plantes (Paris, France) using a portable cardiovascular ultrasound system (Vivid i, GE Healthcare, 9900 Innovation Drive ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were scanned for fluorescence in a Typhoon Trio Scanner 9400 (Control v5.0 + variable Mode Imager-RA 501: PRT<I/06/004, GE Healthcare, Buckingshamshire ...
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bioRxiv - Plant Biology 2021Quote: ... Germany) following manufacturer’s instructions and contaminant genomic DNA was removed using 10 U of DNase I (GE Healthcare, Munich, Germany). The quantity and the quality of RNA was checked using a spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were treated with 5 units RNase I (ART-Seq) per OD260 and monosomes were purified in MicroSpin S-400 columns (GE Healthcare). For both RPF- and RNA-seq ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with RNase I (5U/OD260) and ribosome-protected fragments were isolated via centrifugation through Microspin S-400 HR columns (GE Healthcare) and purified using the RNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2022Quote: Purified NiV F was immobilized to a single flow cell of either (i) a CM5 sensor functionalized with an anti-StreptagII antibody or a NiNTA sensor in a Biacore X100 (GE Healthcare). Binding kinetics for Fabs 4H3 and 1H8 were measured using a CM5 sensor ...
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bioRxiv - Biophysics 2019Quote: ... Purified Nup145NAPD was incubated with trimeric Nup821–854-Nsp1507–718-Nup1591072–1447 and the assembled tetrameric NPC-I complex separated by size-exclusion chromatography on Superdex S200 (GE Healthcare) 20 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 RNAP-based in vitro transcription was performed as described previously.10 The produced λtR1 rut site RNA was treated with DNase I and purified by strong anion-exchange (MonoQ, GE Healthcare) and size-exclusion chromatography (Superdex 75 ...
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bioRxiv - Cancer Biology 2023Quote: ... Recipient mice were allowed to recover for 6 weeks post-transplantation before Cre-LoxP recombination was induced by two intraperitoneal injections of Poly(I)-Poly(C) (300 μg in 200 μl PBS; #27-4732-01 GE Healthcare) with 48 h rest in-between ...
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bioRxiv - Molecular Biology 2023Quote: ... The Triton X-100 was then removed by either (i) binding to a HisTrap 1 mL column (GE Healthcare, Chicago, USA) or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Bioengineering 2022Quote: ... to IL6 was investigated using two approaches: (i) IL6 was directly immobilized on a CM5 biosensor chip surface (GE Healthcare, Uppsala, Sweden) by a standard amine coupling ...
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bioRxiv - Bioengineering 2020Quote: ... Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™, 300 mg I/ml; GE Healthcare) using a CT-scanner (CT ...
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bioRxiv - Microbiology 2024Quote: ... the bacteria were resuspended at an OD600 = 0.5 U in three different nutritional culture conditions: (i) PBSG containing 25 mM HEPES (HyClone GE Healthcare Life Sciences), (ii ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: Binding properties of OT-I TCR interaction with OVA were measured by SPR on a Biacore S200 and T200 (GE Healthcare Life Sciences) using a CM5 sensor chip and by GCI on the WAVEsystem (Creoptix ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I and FANCI was incubated with Strep-Tactin Sepharose High Performance resin (GE Healthcare Life Sciences) for 60 min ...
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bioRxiv - Bioengineering 2019Quote: ... i) Aptamer selection using biotinylated HGM-box that were immobilized on streptavidin-coated magnetic beads (Streptavidin Mag Sepharose™ from GE Healthcare, USA). The bead binding capacity for either HMG-box Pf or HMG-box Hs was estimated to19 pmol/μL beads slurry ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... The denatured DNA was then blotted onto a Hybond LFP membrane using a Minifold I dot blot system (both from GE Healthcare, Piscataway, NJ, USA) followed by a wash with 400 µL TE buffer per well ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µL of 8.1 µM human Notch1fe were loaded on a Superose6 10/300 increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...